Laboratoire de Contrôle des Eaux et Denrées Alimentaires, Centre National de Salmonella, Shigella, et Vibrio spp-Institut Pasteur de Tunis, Tunis, Tunisia.
Foodborne Pathog Dis. 2013 Sep;10(9):813-9. doi: 10.1089/fpd.2012.1466. Epub 2013 Jun 15.
Four hundred and thirty Salmonella isolates, recovered from various food-animal products, were tested for nalidixic acid resistance, plasmid-mediated quinolone resistance, and genetic relationship. One hundred fifteen isolates (113 Salmonella serovar Enteritidis and two Salmonella serovar Typhimurium isolates) of 430 (26.7%) Salmonella isolates exhibited nalidixic acid resistance. Polymerase chain reaction screening for qnrA, qnrB, qnrS, qepA (encoding fluoroquinolones resistance) and rmtB (encoding aminoglycosides resistance) showed that 5 (1.16%) isolates were positive for qnr- and qepA-type genes, and the aac(6')-Ib-cr gene was observed in two (1.7%) Enteritidis isolates concomitantly with qnrA or qnrB. The co-occurrence of qepA and rmtB in one Typhimurium isolate is noteworthy. Pulsed-field gel electrophoresis revealed a high genetic homogeneity of nalidixic-resistant isolates and the persistence of clonal clusters over 4 years in different regions in Tunisia and from various food-animal products. To the best of our knowledge, this is the first report of co-occurrence of qepA and rmtB in a Salmonella strain.
四百三十株从各种食品动物产品中分离得到的沙门氏菌分离株被检测了萘啶酸耐药性、质粒介导的喹诺酮耐药性和遗传关系。在 430 株沙门氏菌分离株中，有 115 株（26.7%）表现出萘啶酸耐药性，其中 113 株为肠炎沙门氏菌血清型，2 株为鼠伤寒沙门氏菌血清型。针对 qnrA、qnrB、qnrS、qepA（编码氟喹诺酮耐药性）和 rmtB（编码氨基糖苷类耐药性）的聚合酶链反应筛选显示，有 5 株（1.16%）分离株携带 qnr-和 qepA 型基因，并且在 2 株肠炎沙门氏菌分离株中同时观察到 aac(6')-Ib-cr 基因与 qnrA 或 qnrB 共表达。在一株鼠伤寒沙门氏菌分离株中同时存在 qepA 和 rmtB 值得注意。脉冲场凝胶电泳显示，耐萘啶酸的分离株具有高度的遗传同质性，并且在突尼斯不同地区和不同食品动物产品中，克隆群在 4 年内持续存在。据我们所知，这是首次在沙门氏菌菌株中同时存在 qepA 和 rmtB 的报道。
