Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University , Shanghai 200240, China.
J Agric Food Chem. 2013 Jul 10;61(27):6693-702. doi: 10.1021/jf401191q. Epub 2013 Jun 26.
Twenty-eight seed samples of 12 Plantago species were investigated for their chemical compositions and anti-inflammatory, cellular antioxidant, and radical scavenging properties. A new UPLC-UV procedure was developed and applied to quantify acteoside and geniposidic acid, the characteristic constituents of the genus Plantago. The amounts of acteoside and geniposidic acid ranged from 0.07 to 15.96 mg/g and from 0.05 to 10.04 mg/g in the tested samples, respectively. Furthermore, 26 compounds were tentatively identified by UPLC/Q-TOF-MS analysis. The Plantago samples significantly differed in their phytochemical compositions. The extracts of Plantago seeds also showed inhibitory effects on LPS-induced IL-1β, IL-6, and COX-2 mRNA expression in RAW 264.7 mouse macrophage cells. Additionally, significant variations were observed among different samples on cellular antioxidant activities in HepG2 cells, as well as DPPH and hydroxyl radical scavenging capacities. The results from this study may be used to promote the use of the genus Plantago in improving human health.
对 12 种车前属植物的 28 个种子样本进行了化学成分、抗炎、细胞抗氧化和自由基清除特性的研究。开发并应用了一种新的 UPLC-UV 方法来定量测定车前属特征成分的毛蕊花糖苷和京尼平苷酸。在测试的样品中，毛蕊花糖苷和京尼平苷酸的含量分别在 0.07 至 15.96 毫克/克和 0.05 至 10.04 毫克/克之间。此外，通过 UPLC/Q-TOF-MS 分析，还初步鉴定了 26 种化合物。车前属植物样品在植物化学成分上有显著差异。车前属种子的提取物对 LPS 诱导的 RAW 264.7 小鼠巨噬细胞中 IL-1β、IL-6 和 COX-2 mRNA 表达也有抑制作用。此外，不同样品在 HepG2 细胞中的细胞抗氧化活性以及 DPPH 和羟基自由基清除能力方面也存在显著差异。本研究的结果可用于促进车前属植物在改善人类健康方面的应用。
