School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
J Agric Food Chem. 2012 Dec 19;60(50):12403-10. doi: 10.1021/jf3042775. Epub 2012 Dec 5.
Fifteen propolis samples collected from different regions of China were investigated and compared for their phytochemical composition and anti-inflammatory and radical scavenging properties. Eleven compounds including caffeic, p-coumaric, ferulic, isoferulic, and 3,4-dimethylcaffeic acids, pinobanksin, chrysin, pinocembrin, galangin, pinobanksin 3-acetate, and caffeic acid phenylethyl ester were quantified for the 15 propolis samples using a UHPLC method, whereas 38 compounds were identified by UPLC/Q-TOF-MS. The 15 propolis samples significantly differed in their total phenolic and total flavonoid contents, as well as their phytochemical profiles. The methanol extracts of propolis also showed significant anti-inflammatory effects in LPS-stimulated RAW 264.7 mouse macrophage cells at 10 μg propolis extract/mL concentration. Additionally, the propolis samples differed in their DPPH, ABTS cation, hydroxyl, and peroxide radical scavenging capacities and ferric reducing abilities. The results from this study may be used to improve the commercial production and consumption of Chinese propolis products.
从中国不同地区采集了 15 个蜂胶样本，对其植物化学成分、抗炎和自由基清除特性进行了研究和比较。采用 UHPLC 法对 15 个蜂胶样品中的 11 种化合物（包括咖啡酸、对香豆酸、阿魏酸、异阿魏酸和 3,4-二甲基咖啡酸、 pinobanksin、白杨素、pinocembrin、乔松素、pinobanksin 3-乙酸酯和咖啡酸苯乙酯）进行了定量分析，而通过 UPLC/Q-TOF-MS 鉴定了 38 种化合物。这 15 个蜂胶样品在总酚和总类黄酮含量以及植物化学成分谱方面存在显著差异。在 10μg 蜂胶提取物/ml 浓度下，蜂胶甲醇提取物对 LPS 刺激的 RAW 264.7 小鼠巨噬细胞也表现出显著的抗炎作用。此外，这些蜂胶样品在 DPPH、ABTS 阳离子、羟基和过氧自由基清除能力以及铁还原能力方面存在差异。本研究结果可用于改善中国蜂胶产品的商业生产和消费。
