School of Sciences, Henan Agricultural University, 450002, China.
Carbohydr Polym. 2013 Jul 25;96(2):411-6. doi: 10.1016/j.carbpol.2013.04.030. Epub 2013 Apr 17.
Aspergillus fumigatus was used to produce polysaccharide using sodium hydroxide pretreated rice straw as the sole carbon source by submerged fermentation. Response surface methodology (RSM) was used to optimize the extraction of polysaccharide from the mycelia of A. fumigatus (PAF). A three-level, three-factor Box-Behnken design (BBD) was applied for experimental design and analyzed the results to obtain the optimal extraction parameters. RSM analysis indicated good correspondence between experimental and predicted values. The optimal conditions for polysaccharide extraction were extraction temperature 50°C, extraction time 6h, ratio of dried mycelia mass to water 0.03. PAF showed excellent antitumor activity in vitro and exert no damage to the normal cells. PAF also showed prominent antitumor activity in vivo against S180 and increased the spleen and thymus index significantly.
烟曲霉被用来通过液体深层发酵以预处理的稻草为唯一碳源生产多糖。采用响应面法（RSM）优化从烟曲霉（PAF）菌丝体中提取多糖。采用三水平三因素 Box-Behnken 设计（BBD）进行实验设计并分析结果以获得最佳提取参数。RSM 分析表明实验值与预测值之间具有良好的一致性。多糖提取的最佳条件为：提取温度 50°C，提取时间 6h，菌丝体干质量与水的比例 0.03。PAF 在体外具有优异的抗肿瘤活性，对正常细胞无损伤。PAF 对 S180 也具有明显的体内抗肿瘤活性，并显著增加脾脏和胸腺指数。
