School of Life Science, Anhui University, Hefei 230039, China.
Int J Biol Macromol. 2013 Jan;52:52-8. doi: 10.1016/j.ijbiomac.2012.09.005. Epub 2012 Sep 13.
Polysaccharides extracted from mushrooms have shown a variety of medical activities, such as antitumor, immunostimulatory and hypoglycemic activity. In this study, characteristics and the antitumor activities of Sarcodon aspratus polysaccharides were investigated for the possibility of application of S. aspratus in health care and medicine. Two polysaccharide fractions (PSAN and PSAA) were extracted and isolated from the mycelium of S. aspratus. The average molecular weight of PSAN and PSAA were approximately 5.6×10(4) Da and 3.83×10(5) Da, respectively. PSAN was composed of L-rhamnose, D-xylose and D-mannose, with molar ratios of 1:10:21; PSAA consisted of L-rhamnose, D-xylose, D-mannose, D-glucose and D-galactose, with molar ratios of 1:39:76:10:21. Both PSAN and PSAA presented high antitumor activity against Hela cells in vitro. At a concentration of 400 mg/L and an exposure time of 24h, the inhibition rates for PSAN and PSAA were 65% and 80%, respectively. PSAN and PSAA exhibited significantly lower cytotoxicity against human normal liver cell line L-02 than Hela tumor cells in comparison with 5-Fu. Polysaccharide extracted from an edible mushroom S. aspratus may be a potential candidate for developing a novel low toxicity antitumor agent.
从蘑菇中提取的多糖显示出多种医学活性，如抗肿瘤、免疫刺激和降血糖活性。在这项研究中，研究了皱盖马鞍菌多糖的特性和抗肿瘤活性，以期将皱盖马鞍菌应用于保健和医药领域。从皱盖马鞍菌菌丝体中提取并分离出两种多糖级分（PSAN 和 PSAA）。PSAN 和 PSAA 的平均分子量分别约为 5.6×10（4）Da 和 3.83×10（5）Da。PSAN 由 L-岩藻糖、D-木糖和 D-甘露糖组成，摩尔比为 1:10:21；PSAA 由 L-岩藻糖、D-木糖、D-甘露糖、D-葡萄糖和 D-半乳糖组成，摩尔比为 1:39:76:10:21。PSAN 和 PSAA 对体外 Hela 细胞均表现出较高的抗肿瘤活性。在浓度为 400mg/L 和暴露时间为 24h 时，PSAN 和 PSAA 的抑制率分别为 65%和 80%。与 5-Fu 相比，PSAN 和 PSAA 对人正常肝细胞系 L-02 的细胞毒性明显低于 Hela 肿瘤细胞。从一种可食用蘑菇皱盖马鞍菌中提取的多糖可能是开发新型低毒性抗肿瘤药物的潜在候选物。
