School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Carbohydr Polym. 2015 Nov 20;133:24-30. doi: 10.1016/j.carbpol.2015.07.013. Epub 2015 Jul 13.
Water-soluble intracellular polysaccharides (IPS) were extracted from cultured mycelia of Phellinus igniarius. The IPS were purified by ethanol fractional precipitation, ion-exchange and size exclusion chromatography in that order. Homogeneous polysaccharide IPSW-1, IPSW-2, IPSW-3, and IPSW-4 were obtained, which molecular characteristics were examined using multiangle laser-light scattering and refractive index detector system. The average molecular weights of them were 34.1, 17.7, 15.1, 21.7kDa, respectively. GC analysis indicated that IPSW-1, IPSW-2 and IPSW-3 all only contained glucose, while IPSW-4 was composed of rhamnose, xylose, mannose, glucose and galactose in a molar ratio of 1.29:1.21:1:43.86:1.86. UV and IR analysis suggested they belonged to α-type of the pyran group and didn't contain protein. These homogeneous polysaccharides could inhibit the growth of SW480 and HepG2 cells to a certain extent in a dose-dependent manner. So they could be beneficial for the further development of a natural carcinoma preventive agent and functional food.
从桑黄发酵菌丝体中提取水溶性胞内多糖（IPS），经乙醇分级沉淀、离子交换和凝胶过滤层析依次分离纯化，得到均一多糖 IPSW-1、IPSW-2、IPSW-3 和 IPSW-4。采用多角度激光散射和折射率检测器系统对它们的分子特性进行了检测。它们的重均分子量分别为 34.1、17.7、15.1 和 21.7 kDa。GC 分析表明 IPSW-1、IPSW-2 和 IPSW-3 均只含有葡萄糖，而 IPSW-4 由鼠李糖、木糖、甘露糖、葡萄糖和半乳糖以摩尔比 1.29:1.21:1:43.86:1.86 组成。UV 和 IR 分析表明它们属于吡喃糖的α型，不含蛋白质。这些均一多糖在一定浓度范围内能以剂量依赖的方式抑制 SW480 和 HepG2 细胞的生长。因此，它们有助于进一步开发天然抗癌剂和功能性食品。
