Sohal R S, Sohal B H, Brunk U T
Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275.
Mech Ageing Dev. 1990 Apr 30;53(3):217-27. doi: 10.1016/0047-6374(90)90040-m.
The general objective of this investigation was to examine the relationship between oxygen free radicals and the aging process. Comparisons of antioxidant defenses were made in six different mammalian species, namely, mouse, rat, guinea pig, rabbit, pig and cow, which range from 3.5 to 30 years in their maximum life span potential (MLSP). Activities of superoxide dismutase (SOD), catalase, and glutathione peroxidase, and concentration of glutathione were measured in the liver, the heart, and the brain. SOD and catalase activities were positively correlated whereas glutathione concentration was negatively correlated with MLSP. Glutathione peroxidase activity exhibited a variable pattern: being positively correlated with MLSP in the brain and negatively correlated in the liver and the heart. In each organ, MLSP was correlated with relatively high levels of one or two of the above antioxidants and low levels of the other antioxidants, indicating the possibility of a compensatory balance among various components of the antioxidant system. No obvious pattern of a relationship was detectable between the overall level of antioxidant defenses and MLSP among the mammalian species examined. The implications of this finding concerning the role of oxidative stress in the aging process and the free radical hypothesis of aging are discussed.
本研究的总体目标是考察氧自由基与衰老过程之间的关系。对六种不同的哺乳动物进行了抗氧化防御能力的比较，这六种动物分别是小鼠、大鼠、豚鼠、兔子、猪和牛，它们的最大寿命潜能（MLSP）从3.5岁到30岁不等。测定了肝脏、心脏和大脑中超氧化物歧化酶（SOD）、过氧化氢酶和谷胱甘肽过氧化物酶的活性以及谷胱甘肽的浓度。SOD和过氧化氢酶活性呈正相关，而谷胱甘肽浓度与MLSP呈负相关。谷胱甘肽过氧化物酶活性呈现出一种可变模式：在大脑中与MLSP呈正相关，在肝脏和心脏中与MLSP呈负相关。在每个器官中，MLSP与上述一种或两种抗氧化剂的相对高水平以及其他抗氧化剂的低水平相关，这表明抗氧化系统各组分之间可能存在代偿平衡。在所研究的哺乳动物物种中，未检测到抗氧化防御总体水平与MLSP之间存在明显的关系模式。讨论了这一发现对氧化应激在衰老过程中的作用以及衰老自由基假说的意义。
