AcSIR-Central Salt & Marine Chemicals Research Institute, G. B. Marg, Bhavnagar-364002, Gujarat, India.
Chem Commun (Camb). 2013 Aug 7;49(61):6849-51. doi: 10.1039/c3cc42829j. Epub 2013 Jun 14.
DNA from salmon testes was solubilised in two bio-based ionic liquids up to 3.5% w/w in 6 h. No structural degradation of the molecule was observed for the sample solubilised in choline-indole-3-acetate (chol-IAA). However, the molecule was found to be denatured in choline-indole-3-butyrate (chol-IBA). The structural and chemical stability of the DNA molecules after six months of storage in the former was established. Further recyclability of the ionic liquid with very high yield (90-95%) for consecutive reuse in the redissolution of DNA was demonstrated.
从三文鱼睾丸中提取的 DNA 在两种生物基离子液体中溶解，6 小时内最高可达 3.5%（重量/重量）。在胆碱吲哚-3-乙酸酯（胆碱-IAA）中溶解的样品中，没有观察到分子的结构降解。然而，在胆碱吲哚-3-丁酸酯（胆碱-IBA）中，发现该分子发生了变性。在前者中储存六个月后，确定了 DNA 分子的结构和化学稳定性。进一步证明了离子液体的高回收率（90-95%），可连续重复用于 DNA 的再溶解。
