University of Sheffield and Sheffield Teaching Hospitals NHS Trust, Royal Hallamshire Hospital, Sheffield, UK.
BJU Int. 2013 Sep;112(5):674-85. doi: 10.1111/bju.12186. Epub 2013 Jun 14.
To identify candidate materials which have sufficient potential to be taken forward for an in vivo tissue-engineering approach to restoring the tissue structure of the pelvic floor in women with stress urinary incontinence (SUI) or pelvic organ prolapse (POP).
Oral mucosal fibroblasts were seeded onto seven different scaffold materials, AlloDerm ( LifeCell Corp., Branchburg, NJ, USA), cadaveric dermis, porcine dermis, polypropylene, sheep forestomach, porcine small intestinal submucosa (SIS) and thermoannealed poly(L) lactic acid (PLA) under both free and restrained conditions. The scaffolds were assessed for: cell attachment using AlamarBlue and 4,6-diamidino-2-phenylindole (DAPI); contraction using serial photographs; and extracellular matrix production using Sirius red staining, immunostaining and scanning electron microscopy. Finally the biomechanical properties of all the scaffolds were assessed.
Of the seven, there were two biodegradable scaffolds, synthetic PLA and natural SIS, which supported good cell attachment and proliferation. Immunostaining confirmed the presence of collagen I, III and elastin which was highest in SIS and PLA. The mechanical properties of PLA were closest to native tissue with an ultimate tensile strength of 0.72 ± 0.18 MPa, ultimate tensile strain 0.53 ± 0.16 and Young's modulus 4.5 ± 2.9 MPa. Scaffold restraint did not have a significant impact on the above properties in the best scaffolds.
These data support both PLA and SIS as good candidate materials for use in making a tissue-engineered repair material for SUI or POP.
确定具有足够潜力的候选材料，以便进一步采用体内组织工程方法来修复患有压力性尿失禁（SUI）或盆腔器官脱垂（POP）的女性盆底组织结构。
将口腔黏膜成纤维细胞接种到七种不同的支架材料上，包括 AlloDerm（LifeCellCorp.，新泽西州布兰奇伯勒）、尸体真皮、猪真皮、聚丙烯、绵羊胃网膜、猪小肠黏膜下层（SIS）和热退火聚（L）乳酸（PLA），在自由和约束条件下进行接种。评估支架的以下指标：使用 AlamarBlue 和 4,6-二脒基-2-苯基吲哚（DAPI）进行细胞附着；使用连续照片进行收缩；使用天狼星红染色、免疫染色和扫描电子显微镜进行细胞外基质产生。最后评估所有支架的生物力学特性。
在这七种支架中，有两种可生物降解的支架，即合成 PLA 和天然 SIS，它们支持良好的细胞附着和增殖。免疫染色证实了胶原 I、III 和弹性蛋白的存在，其中 SIS 和 PLA 中的含量最高。PLA 的机械性能最接近天然组织，其极限拉伸强度为 0.72±0.18MPa，极限拉伸应变 0.53±0.16，杨氏模量 4.5±2.9MPa。在最好的支架中，支架约束对上述性能没有显著影响。
这些数据支持 PLA 和 SIS 作为用于制造治疗 SUI 或 POP 的组织工程修复材料的候选材料。
