Valenzuela Nicole M, Reed Elaine F
Department of Pathology and Laboratory Medicine, UCLA Immunogenetics Center, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Methods Mol Biol. 2013;1034:41-70. doi: 10.1007/978-1-62703-493-7_2.
Until recently, allograft rejection was thought to be mediated primarily by alloreactive T cells. Consequently, immunosuppressive approaches focused on inhibition of T cell activation. While short-term graft survival has significantly improved and rejection rates have dropped, acute rejection has not been eliminated and chronic rejection remains the major threat to long-term graft survival. Increased attention to humoral immunity in experimental systems and in the clinic has revealed that donor specific antibodies (DSA) can mediate and promote acute and chronic rejection. Herein, we detail the effects of alloantibody, particularly HLA antibody, binding to graft vascular and other cells, and briefly summarize the experimental methods used to assess such outcomes.
直到最近，同种异体移植排斥反应一直被认为主要是由同种异体反应性T细胞介导的。因此，免疫抑制方法主要集中在抑制T细胞活化上。虽然短期移植物存活率有了显著提高，排斥反应率也有所下降，但急性排斥反应并未消除，慢性排斥反应仍然是长期移植物存活的主要威胁。在实验系统和临床中，对体液免疫的关注度增加，这表明供体特异性抗体（DSA）可以介导并促进急性和慢性排斥反应。在此，我们详细阐述同种异体抗体，特别是HLA抗体，与移植血管及其他细胞结合的效应，并简要总结用于评估此类结果的实验方法。
