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通过使用新型全息相关速度测量技术优化搅拌式生物反应器。

Optimisation of a stirred bioreactor through the use of a novel holographic correlation velocimetry flow measurement technique.

机构信息

Department of Mechanical and Aerospace Engineering, Monash University, Melbourne, Victoria, Australia.

出版信息

PLoS One. 2013 Jun 11;8(6):e65714. doi: 10.1371/journal.pone.0065714. Print 2013.

DOI:10.1371/journal.pone.0065714
PMID:23776534
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3679106/
Abstract

We describe a method for measuring three dimensional (3D) velocity fields of a fluid at high speed, by combining a correlation-based approach with in-line holography. While this method utilizes tracer particles contained within the flow, our method does not require the holographic reconstruction of 3D images. The direct flow reconstruction approach developed here allows for measurements at seeding densities in excess of the allowable levels for techniques based on image or particle reconstruction, thus making it suited for biological flow measurement, such as the flow in bioreactor. We outline the theory behind our method, which we term Holographic Correlation Velocimetry (HCV), and subsequently apply it to both synthetic and laboratory data. Moreover, because the system is based on in-line holography, it is very efficient with regard to the use of light, as it does not rely on side scattering. This efficiency could be utilized to create a very high quality system at a modest cost. Alternatively, this efficiency makes the system appropriate for high-speed flows and low exposure times, which is essential for imaging dynamic systems.

摘要

我们描述了一种结合相关方法和线全息术来测量高速流场三维(3D)速度场的方法。虽然该方法利用了流场中的示踪粒子,但我们的方法不需要对 3D 图像进行全息重建。这里开发的直接流重建方法允许在超过基于图像或粒子重建技术的允许密度下进行测量,因此非常适合生物流动测量,例如生物反应器中的流动。我们概述了我们称之为全息相关速度测量(HCV)的方法背后的理论,随后将其应用于合成数据和实验室数据。此外,由于该系统基于线全息术,因此在光的使用方面非常高效,因为它不依赖于侧向散射。这种效率可以用来以适中的成本创建一个非常高质量的系统。或者,这种效率使得该系统适用于高速流和短曝光时间,这对于成像动态系统至关重要。

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