几内亚猴面包树（野生）茎皮对尼日利亚一些临床分离株的抗菌潜力。

Antimicrobial potential of Dialium guineense (Wild.) stem bark on some clinical isolates in Nigeria.

作者信息

Olajubu Fa, Akpan I, Ojo DA, Oluwalana Sa

机构信息

Department of Medical Microbiology and Parasitology, Olabisi Onabanjo University Teaching Hospital, Sagamu, Nigeria.

出版信息

Int J Appl Basic Med Res. 2012 Jan;2(1):58-62. doi: 10.4103/2229-516X.96811.

DOI:10.4103/2229-516X.96811

PMID:23776811

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3657983/

Abstract

CONTEXT

The persistent increase in the number of antibiotic-resistant strains of microorganisms has led to the development of more potent but also more expensive antibiotics. In most developing countries of the world these antibiotics are not readily affordable, thus making compliance difficult. This calls for research into alternative sources of antimicrobials. Dialium guineense is a shrub of the family Leguminosae. Its stem bark is used for the treatment of cough, toothache, and bronchitis.

AIMS

Despite the acclaimed efficacy of D guineense, there is no scientific evidence in its support. This work was carried out to assess the antimicrobial activity of D guineense in vitro against some clinical isolates.

MATERIALS AND METHODS

D guineense stem bark was collected and 50 gm of air-dried and powdered stem bark of the plant was soaked for 72 hours in 1 l of each of the six solvents used in this study. Each mixture was refluxed, agitated at 200 rpm for 1 hour, filtered using Whatman No. 1 filter paper and, finally, freeze dried. The extracts were then tested for antimicrobial activity using the agar diffusion method.

RESULTS

The highest percentage yield of 23.2% was obtained with ethanol. Phytochemical screening showed that D guineense contains anthraquinone, alkaloids, flavonoids, tannins, and saponins. The antimicrobial activity of the extracts revealed a broad spectrum of activity, with Salmonella typhi and Staphylococcus aureusa showing the greatest zones of inhibition (18.0 mm). Only Candida albicans among the fungi tested was inhibited by the extract. The greatest zone of inhibition among the fractions was 16.0 mm. D guineense exhibited bactericidal activity at the 7th and 9th hours against Streptococcus pneumoniae and S. aureus 25923 while the 10th hour against S. typhi and C. albicans. The greatest activity was noted against S pneumoniae, where there was reduced viable cell count after 6 hours of exposure.

CONCLUSION

Stem bark extract of D guineense (Wild.) has the potential to be developed into an antimicrobial agent.

摘要

背景

微生物对抗生素耐药菌株数量的持续增加导致了更高效但也更昂贵的抗生素的研发。在世界上大多数发展中国家，这些抗生素难以负担得起，因此难以保证患者的依从性。这就需要研究抗菌药物的替代来源。几内亚猴耳环（Dialium guineense）是豆科的一种灌木。其茎皮用于治疗咳嗽、牙痛和支气管炎。

目的

尽管几内亚猴耳环的疗效广受赞誉，但尚无科学证据支持。本研究旨在评估几内亚猴耳环在体外对一些临床分离株的抗菌活性。

材料与方法

采集几内亚猴耳环的茎皮，将50克风干并磨成粉末的植物茎皮分别浸泡在本研究使用的六种溶剂中的每一种1升中72小时。每种混合物进行回流，以200转/分钟搅拌1小时，用1号滤纸过滤，最后冷冻干燥。然后使用琼脂扩散法测试提取物的抗菌活性。

结果

乙醇提取物的产率最高，为23.2%。植物化学筛选表明，几内亚猴耳环含有蒽醌、生物碱、黄酮类、单宁和皂苷。提取物的抗菌活性显示出广泛的活性谱，伤寒沙门氏菌和金黄色葡萄球菌显示出最大的抑菌圈（18.0毫米）。在所测试的真菌中，只有白色念珠菌被提取物抑制。各馏分中最大抑菌圈为16.0毫米。几内亚猴耳环在第7小时和第9小时对肺炎链球菌和金黄色葡萄球菌25923表现出杀菌活性，而在第10小时对伤寒沙门氏菌和白色念珠菌表现出杀菌活性。对肺炎链球菌的活性最强，暴露6小时后活菌数减少。