Sampaio Thaíse Pd, Cartaxo-Furtado Nathália Ao, de Medeiros Ana Cd, Alves Harley S, Rosalen Pedro L, Pereira Jozinete V
Department of Dentistry, State University of Paraíba, Campina Grande, Paraíba, Brazil, e-mail:
Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
J Contemp Dent Pract. 2017 May 1;18(5):392-398. doi: 10.5005/jp-journals-10024-2053.
The aim of this study is to evaluate the in vitro antimicrobial activity of plant extracts and chemical fractions of Sideroxylon obtusifolium T.D. Penn on Streptococcus mutans, Streptococcus oralis, Streptococcus salivarius, Streptococcus parasanguinis, and Candida albicans as well as to identify the chemical classes found in the bioactive extracts possessing better activity.
Freeze-dried hydroalcoholic extracts of the bark and leaves (LC and LF respectively) and ethanol extracts of bark and leaves (EC and EF respectively) of S. obtusifolium were assessed for antimicrobial potential by determining the minimum inhibitory concentration (MIC), minimum bactericidal concentration, and minimum fungicidal concentration (MFC). The tests were performed by microdilu-tion method (in triplicate) in three independent experiments. Phytochemical characterization was performed by quantification of total polyphenols, total flavonoids, and condensed tannins.
The EC extract presented weak antimicrobial potential on the growth of S. mutans (MIC = 1000 μg/mL); all extracts showed moderate inhibitory activity on the growth of C. albicans (MIC = 500 μg/mL). The dichloromethane and n-butanol fractions of LF extracts showed moderate growth inhibitory activity (MIC = 250 μg/mL) on C. albicans and fungicide potential (MFC/MIC = 2). The phytochemical characterization revealed a predominance of total polyphenols (EC = 29.23%; LC = 25.98%) and condensed tannins (LC = 38.84%; LF = 17.78%).
The dichloromethane and n-butanol fractions of S. obtusifolium LF extract showed antifungal activity against C. albicans, with the potential for bioprospection of phytocom-pounds for the treatment of periodontal fungal diseases caused by this microorganism. The effect may be related to phyto-chemical compounds from the polyphenol and condensed tannin classes.
Research for new oral microbial disease treatment alternatives in bioactive compounds from medicinal plants is of clinical relevance and scientific interest since many therapeutic antifungal agents do not obtain the effectiveness expected due to microbial resistance, or to adverse effects on human tissues.
本研究旨在评估钝叶铁线子植物提取物及其化学组分对变形链球菌、口腔链球菌、唾液链球菌、血链球菌和白色念珠菌的体外抗菌活性，并鉴定具有较好活性的生物活性提取物中的化学类别。
通过测定最低抑菌浓度（MIC）、最低杀菌浓度和最低杀真菌浓度（MFC），评估钝叶铁线子树皮和树叶的冻干水醇提取物（分别为LC和LF）以及树皮和树叶的乙醇提取物（分别为EC和EF）的抗菌潜力。试验采用微量稀释法（一式三份），进行三个独立实验。通过对总多酚、总黄酮和缩合单宁的定量分析进行植物化学特征鉴定。
EC提取物对变形链球菌的生长表现出较弱的抗菌潜力（MIC = 1000 μg/mL）；所有提取物对白色念珠菌的生长均表现出中等抑制活性（MIC = 500 μg/mL）。LF提取物的二氯甲烷和正丁醇馏分对白色念珠菌表现出中等生长抑制活性（MIC = 250 μg/mL）和杀真菌潜力（MFC/MIC = 2）。植物化学特征鉴定显示总多酚含量较高（EC = 29.23%；LC = 25.98%），缩合单宁含量较高（LC = 38.84%；LF = 17.78%）。
钝叶铁线子LF提取物的二氯甲烷和正丁醇馏分对白色念珠菌具有抗真菌活性，具有对该微生物引起的牙周真菌病进行植物化合物生物勘探的潜力。其作用可能与多酚类和缩合单宁类植物化学化合物有关。
由于许多治疗性抗真菌药物因微生物耐药性或对人体组织的不良反应而未达到预期效果，因此从药用植物中寻找新的口腔微生物疾病治疗替代生物活性化合物的研究具有临床相关性和科学意义。
