Department of Materials Engineering, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Anal Chem. 2013 Jul 16;85(14):6633-8. doi: 10.1021/ac4001018. Epub 2013 Jun 28.
In this study, we have proposed and demonstrated experimentally a novel monitoring device of single mouse embryo activity after in vitro fertilization (IVF) using a semiconductor-based field effect transistor (FET). The FET biosensor realized to detect it noninvasively, quantitatively, and continuously by change of hydrogen ions with positive charges, which were induced by dissolved carbon dioxide due to cellular respiration activity during cleavage. The electrical signal of FET biosensor should become an effective indication to evaluate objectively single embryo activity as its morphology is observed subjectively after IVF. The platform based on the FET biosensor will contribute to promote elective single embryo transfer (eSET) in human assisted reproductive technology (ART).
在这项研究中，我们提出并实验证明了一种使用基于半导体的场效应晶体管（FET）对体外受精（IVF）后单个胚胎活动进行新型监测的设备。FET 生物传感器通过细胞呼吸活动过程中产生的正电荷氢离子的变化来实现非侵入性、定量和连续的检测，这些氢离子是由溶解的二氧化碳引起的。FET 生物传感器的电信号将成为评估单个胚胎活动的有效指标，因为在 IVF 之后，其形态是主观观察的。基于 FET 生物传感器的平台将有助于推动人类辅助生殖技术（ART）中的选择性单胚胎移植（eSET）。
