Laboratory of Molecular Cytogenetics, Department of Ruminant Science, The West Pomeranian University of Technology, Judyma 10, 71-460, Szczecin, Poland.
J Appl Genet. 2013 Aug;54(3):305-8. doi: 10.1007/s13353-013-0155-z. Epub 2013 Jun 19.
The insulin-like growth factor 1 (IGF-1) is considered to be a factor that mainly regulates growth, differentiation, and the maintenance of various function in numerous tissues through binding to a family of transmembrane tyrosine kinase receptors, signaling primarily through the insulin-like growth factor 1 receptor (IGF-1R) encoded by the IGF1R gene. The objectives of the present study were to estimate the allele and genotype frequencies of the IGF1R/MspI (silent mutation within exon 12) and the IGF1R/TaqI (within the 3' untranslated region, 3'UTR) gene polymorphisms in beef cattle and to determine associations between these polymorphisms and growth traits. In a preliminary study on 310 Angus calves, association analyses with three production traits (birth weight, BWT; weaning weight adjusted to 210 days, WWT210; and average daily gain, ADG) were conducted. The GG genotype of the IGF1R/e12/MspI polymorphism was significantly associated (P ≤ 0.05) with a higher WWT210 (+5.06 kg) compared to the AG genotype. Polymorphism within the 3'UTR had no significant effect on growth traits. The effect of combined genotypes was also examined. At WWT(210), calves with the GG/AA and GG/AG combinations were heavier than calves with the AG/AA and AG/AG combined genotypes (P ≤ 0.05). To our knowledge, this is the first report of a polymorphism within the coding region of the Bos taurus IGF1R gene.
胰岛素样生长因子 1（IGF-1）被认为是一种主要通过结合一组跨膜酪氨酸激酶受体来调节生长、分化和维持各种组织功能的因子，主要通过 IGF1R 基因编码的胰岛素样生长因子 1 受体（IGF-1R）信号传递。本研究的目的是估计 IGF1R/MspI（外显子 12 内的沉默突变）和 IGF1R/TaqI（3'非翻译区，3'UTR）基因多态性在肉牛中的等位基因和基因型频率，并确定这些多态性与生长性状之间的关系。在对 310 头安格斯小牛进行的初步研究中，对三个生产性状（出生体重，BWT；调整至 210 天的断奶体重，WWT210；和平均日增重，ADG）进行了关联分析。IGF1R/e12/MspI 多态性的 GG 基因型与较高的 WWT210（+5.06kg）显著相关（P≤0.05），与 AG 基因型相比。3'UTR 内的多态性对生长性状没有显著影响。还检查了组合基因型的影响。在 WWT（210）时，GG/AA 和 GG/AG 组合的小牛比 AG/AA 和 AG/AG 组合的小牛更重（P≤0.05）。据我们所知，这是首次报道牛 IGF1R 基因编码区的多态性。
