Bienvenu Emile, Hoffmann Kurt-Jürgen, Ashton Michael, Kayumba Pierre Claver
Unit for Pharmacokinetics and Drug Metabolism, Department of Pharmacology, Sahlgrenska Academy at University of Gothenburg, Göteborg, Sweden.
Biomed Chromatogr. 2013 Nov;27(11):1554-9. doi: 10.1002/bmc.2959. Epub 2013 Jun 18.
Owing to heterogeneity in therapeutic response, efavirenz is of research and clinical interest. There is a need to quantitate it using noncostly and selective methods. A method for efavirenz quantitation in plasma containing HIV and tuberculosis drugs was developed. Chromatographic separation was carried out using a C18 column. The mobile phase consisted of 0.1% formic acid and acetonitrile, and was pumped at a flow rate of 0.3 mL/min. Efavirenz and ritonavir (internal standard) were monitored at 247 nm. Plasma proteins were precipitated by centrifugation. The analysis time was 6 min. The response was linear (r = 0.9997). The accuracy ranged between 98 and 115% (intraday) and between 99 and 117% (interday). The precision ranged from 1.670 to 4.087% (intraday) and from 3.447 to 13.347% (interday). Recovery ranged from 98 to 132%. Stability ranged between 99 and 123%. The selectivity was proven by analysis of drugs used for the management of HIV/AIDS and tuberculosis. Plasma sample analysis showed an efavirenz retention time of 5.57 min and a peak plasma concentration of 2.4 µg/mL occurring at 2 h. This method is rapid and selective, and thus suitable for monitoring efavirenz in patients with HIV/AIDS alone or co-infected with tuberculosis in a less resourced setting.
由于治疗反应存在异质性，依非韦伦具有研究和临床意义。需要使用低成本且具选择性的方法对其进行定量分析。已开发出一种用于定量分析含有抗艾滋病毒和抗结核药物的血浆中依非韦伦的方法。采用C18柱进行色谱分离。流动相由0.1%甲酸和乙腈组成，以0.3 mL/min的流速泵送。在247 nm处监测依非韦伦和利托那韦（内标）。通过离心沉淀血浆蛋白。分析时间为6分钟。响应呈线性（r = 0.9997）。准确度在98%至115%（日内）和99%至117%（日间）之间。精密度在1.670%至4.087%（日内）和3.447%至13.347%（日间）之间。回收率在98%至132%之间。稳定性在99%至123%之间。通过对用于治疗艾滋病毒/艾滋病和结核病的药物进行分析证明了其选择性。血浆样本分析显示依非韦伦的保留时间为5.57分钟，血浆峰值浓度为2.4 µg/mL，出现在2小时时。该方法快速且具选择性，因此适用于在资源较少的环境中监测单纯感染艾滋病毒/艾滋病或合并感染结核病患者体内的依非韦伦。
