Dubovi Edward J, Hawkins Melissa, Griffin Robert A, Johnson Donna J, Ostlund Eileen N
Animal Health Diagnostic Center, Cornell University, Ithaca, NY, USA.
J Vet Diagn Invest. 2013 Jul;25(4):490-2. doi: 10.1177/1040638713489982. Epub 2013 Jun 18.
Three aborted canine fetuses were submitted to the Animal Health Diagnostic Center at Cornell University in November 2011 and September 2012 for diagnostic workups to determine the causes of the reproductive difficulties. Histological assessments of the sampled tissues were inconclusive due to the autolysis. Tests to detect bacterial causes of the abortions were also negative. Virus isolation testing on pooled tissues from the fetuses identified a cytopathogenic agent in cell cultures. Fluorescent antibody tests on the infected cells gave a positive reaction for Bluetongue virus, and subsequent serotype specific reverse transcription polymerase chain reaction assays identified the isolates as Bluetongue virus serotype 11. The current report describes the isolation of Bluetongue virus from dogs unrelated to contaminated vaccines and in the absence of a raw meat diet.
2011年11月和2012年9月，三只流产的犬胎儿被送到康奈尔大学动物健康诊断中心进行诊断检查，以确定繁殖困难的原因。由于组织自溶，对采样组织的组织学评估没有得出明确结论。检测流产细菌病因的试验也呈阴性。对胎儿混合组织进行的病毒分离检测在细胞培养物中发现了一种细胞致病因子。对感染细胞进行的荧光抗体检测对蓝舌病毒呈阳性反应，随后的血清型特异性逆转录聚合酶链反应分析确定分离株为蓝舌病毒血清型11。本报告描述了在与受污染疫苗无关且没有生食饮食的犬只中分离出蓝舌病毒的情况。
