Brown C C, Rhyan J C, Grubman M J, Wilbur L A
Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratories, APHIS-USDA, Greenport, NY, USA.
Vet Pathol. 1996 May;33(3):337-40. doi: 10.1177/030098589603300311.
Six female dogs (four pregnant and two nonpregnant) were inoculated with bluetongue virus (BTV), serotype 11. Pregnant animals and one nonpregnant dog received 5.5-6.3 log10 of cell culture-adapted virus. The other nonpregnant dog received a modified live vaccine contaminated with bluetongue virus. The non-pregnant animals never became clinically ill and were euthanatized 35 days post-inoculation. Three of the four pregnant dogs aborted, and all four died or were euthanatized 5-10 days post-inoculation. The predominant pathologic feature in the adults was severe pulmonary edema. Various tissues from the bitches and fetuses were examined by in situ hybridization using a digoxigenin-labeled probe corresponding to the nonstructural protein-1 gene of BTV-17. By this technique, viral nucleic acid was detected predominantly in endothelial cells of lung of all four dogs, with lesser amounts in capillaries of uterus, spleen, and kidney in some of the dogs. In two adult dogs, bluetongue viral nucleic acid was detected in mononuclear cells of the periarteriolar lymphoid sheaths of spleen. There was minimal staining of capillaries in placenta in three of the five fetuses examined. There was no viral nucleic acid detected in any of the other fetal tissues.
六只雌性犬（四只怀孕，两只未怀孕）接种了血清型11的蓝舌病毒（BTV）。怀孕的动物和一只未怀孕的犬接种了5.5 - 6.3 log10的细胞培养适应病毒。另一只未怀孕的犬接种了被蓝舌病毒污染的改良活疫苗。未怀孕的动物从未出现临床疾病，并在接种后35天实施安乐死。四只怀孕犬中有三只流产，所有四只均在接种后5 - 10天死亡或被安乐死。成年犬的主要病理特征是严重肺水肿。使用与BTV - 17非结构蛋白-1基因对应的地高辛标记探针，通过原位杂交对母犬和胎儿的各种组织进行检查。通过该技术，在所有四只犬的肺内皮细胞中主要检测到病毒核酸，在一些犬的子宫、脾脏和肾脏的毛细血管中检测到的量较少。在两只成年犬中，在脾脏动脉周围淋巴鞘的单核细胞中检测到蓝舌病毒核酸。在所检查的五只胎儿中，有三只胎儿的胎盘毛细血管染色最少。在任何其他胎儿组织中均未检测到病毒核酸。
