Chauhan H C, Biswas S K, Chand K, Rehman W, Das B, Dadawala A I, Chandel B S, Kher H N, Mondal B
Rev Sci Tech. 2014 Dec;33(3):803-12. doi: 10.20506/rst.33.3.2319.
Abortions and stillbirths were noticed in pregnant goats on a farm in the state of Gujarat, India. About 50% of the pregnant goats aborted or gave birth to dead kids. Bluetongue virus (BTV) antibody in the sera of affected goats was detected using a competitive enzyme-linked immunosorbent assay (ELISA). Viral antigen in the blood of these goats and in the aborted fetal spleens was detected using a sandwich ELISA. Two viruses (SKN-9, SKN-10) were isolated in cell culture from aborted fetal spleens and were confirmed as Orbivirus by demonstration of ten bands in RNA polyacrylamide gel electrophoresis and identified as BTV-1 by sequencing of the VP2 gene. Sequence analyses revealed thatthese isolates were very closely related to a BTV-1 (strain SKN-8) isolated from Culicoides vectors captured on the same farm one month after the occurrence of abortion. Isolation of BTV-1 from fetuses is probably evidence of transplacental transmission of the wild-type strain, because attenuated or laboratory-adapted BTV-1 strains have never been used in this region. This may have important implications in the epidemiology of bluetongue, considering the presence of many BTV serotypes in India.
在印度古吉拉特邦的一个农场里，怀孕山羊出现了流产和死产情况。约50%的怀孕山羊流产或产下死胎。采用竞争酶联免疫吸附测定（ELISA）检测了患病山羊血清中的蓝舌病病毒（BTV）抗体。使用夹心ELISA检测了这些山羊血液和流产胎儿脾脏中的病毒抗原。从流产胎儿脾脏的细胞培养物中分离出两种病毒（SKN - 9、SKN - 10），通过RNA聚丙烯酰胺凝胶电泳显示十条带证实为环状病毒属，并通过VP2基因测序鉴定为BTV - 1。序列分析表明，这些分离株与流产发生一个月后在同一农场捕获的库蠓载体中分离出的BTV - 1（SKN - 8株）密切相关。从胎儿中分离出BTV - 1可能是野生型毒株经胎盘传播的证据，因为该地区从未使用过减毒或实验室适应的BTV - 1毒株。考虑到印度存在多种BTV血清型，这可能对蓝舌病的流行病学具有重要意义。
