Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe 605-0047, Japan.
Anticancer Res. 2013 Jul;33(7):2867-73.
A novel model system to study the cellular, molecular, and genetic characteristics of breast cancer stem cells is needed. Personalized prognostic models are indispensable for assessing the effect of so-called tailor-made adjuvant therapy.
Surgically-extirpated tissues were dispersed with Dispase and cultured in commonly used medium. The expression of tumor markers was detected with immunohistochemistry, and gene expression profiles of tissues and cells were analyzed using the Agilent Human Microarray.
We established a primary culture, which exhibited mesenchymal morphology. The gene expression profile of the primary culture revealed that the cells underwent epithelial-mesenchymal transition and had cancer stem cell properties.
A primary culture of breast cancer cells can be easily established and could be used for studying breast cancer stem cells and assessing treatment of a patient.
需要一种新的模型系统来研究乳腺癌干细胞的细胞、分子和遗传特征。个性化的预后模型对于评估所谓的定制辅助治疗的效果是必不可少的。
用Dispase 分散手术切除的组织，并在常用培养基中培养。用免疫组织化学检测肿瘤标志物的表达，并使用 Agilent Human Microarray 分析组织和细胞的基因表达谱。
我们建立了一个表现出间充质形态的原代培养物。原代培养物的基因表达谱表明，细胞经历了上皮-间充质转化，并具有癌症干细胞特性。
可以很容易地建立乳腺癌细胞的原代培养物，可用于研究乳腺癌干细胞和评估患者的治疗效果。
