Minafra Luigi, Bravatà Valentina, Forte Giusi Irma, Cammarata Francesco Paolo, Gilardi Maria Carla, Messa Cristina
Contrada Pietrapollastra-Pisciotto, 90015-Cefalù, PA, Italy.
Anticancer Res. 2014 May;34(5):2173-83.
BACKGROUND/AIM: Epithelial-mesenchymal transition (EMT) is a process co-opted by cancer cells to invade and form metastases. In the present study we analyzed gene expression profiles of primary breast cancer cells in culture in order to highlight genes related to EMT.
Microarray expression analysis of primary cells isolated from a specimen of a patient with an infiltrating ductal carcinoma of the breast was performed. Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) analyses validated microarray gene expression trends.
Thirty-six candidate genes were selected and used to generate a molecular network displaying the tight relationship among them. The most significant Gene Ontology biological processes characterizing this network were involved in cell migration and motility.
Our data revealed the involvement of new genes which displayed tight relationships among them, suggesting a molecular network in which they could contribute to control of EMT in breast cancer. This study may offer a basis for understanding complex mechanisms which regulate breast cancer progression and for designing individualized anticancer therapies.
背景/目的：上皮-间质转化（EMT）是癌细胞用于侵袭和形成转移的一个过程。在本研究中，我们分析了培养的原发性乳腺癌细胞的基因表达谱，以突出与EMT相关的基因。
对从一名乳腺浸润性导管癌患者的标本中分离出的原代细胞进行了微阵列表达分析。实时定量逆转录PCR（qRT-PCR）分析验证了微阵列基因表达趋势。
选择了36个候选基因并用于生成一个显示它们之间紧密关系的分子网络。表征该网络的最显著的基因本体生物学过程涉及细胞迁移和运动。
我们的数据揭示了新基因的参与，这些基因之间显示出紧密的关系，提示存在一个分子网络，其中它们可能有助于控制乳腺癌中的EMT。本研究可能为理解调节乳腺癌进展的复杂机制以及设计个体化抗癌治疗提供基础。
