Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Biomed Res Int. 2013;2013:590730. doi: 10.1155/2013/590730. Epub 2013 May 26.
The data on the biocompatibility of naked gold nanoparticles (GNPs) are scarce, and their interpretation is controversial. We studied the acute (1 day) and subchronic (5 days) effects of GNPs (10 and 50 nm diameter) on expression of interleukin-1 beta (IL-1 β ), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF- α ) in the liver and kidneys of rats. In the liver, the GNPs of both sizes (10 and 50 nm) significantly increased the cytokines gene expression on day 1 which was subsided on day 5; the GNPs of 50 nm size produced more severe inflammatory response as compared to smaller sized GNPs. In the kidney, the GNPs did not produce any significant change in the expression of IL-1 β . Although the gene expression of IL-6 and TNF- α was not affected by GNPs of 10 nm size, 50 nm GNPs significantly increased the expression of IL-6 and TNF- α in the kidneys of rats on day 1 after treatment which returned to normalcy on day 5. These findings indicate the possible immunocompatibility of medium sized GNPs as they caused only a transient acute phase increase in proinflammatory cytokines expression followed by their normalcy during the repeated exposure.
关于裸露金纳米粒子（GNPs）的生物相容性的数据很少，其解释也存在争议。我们研究了 GNPs（直径为 10 和 50nm）对大鼠肝脏和肾脏中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）表达的急性（1 天）和亚慢性（5 天）影响。在肝脏中，两种大小（10 和 50nm）的 GNPs 在第 1 天均显著增加了细胞因子的基因表达，第 5 天则有所下降；与较小尺寸的 GNPs 相比，50nm 尺寸的 GNPs 产生了更严重的炎症反应。在肾脏中，GNPs 对 IL-1β的表达没有产生任何显著变化。尽管 10nm 尺寸的 GNPs 对 IL-6 和 TNF-α的基因表达没有影响，但 50nm GNPs 在治疗后第 1 天显著增加了大鼠肾脏中 IL-6 和 TNF-α的表达，第 5 天恢复正常。这些发现表明中等尺寸的 GNPs 可能具有免疫相容性，因为它们仅在反复暴露期间引起短暂的急性前炎症细胞因子表达增加，随后恢复正常。
