Faculty of Sport Sciences, Waseda University, 2-579-15 Mikajima, Tokorozawa, Saitama 359-1192, Japan.
Life Sci. 2013 Aug 14;93(5-6):208-13. doi: 10.1016/j.lfs.2013.06.007. Epub 2013 Jun 16.
The purpose of this study was to investigate the effects of alternate-day fasting (ADF) on insulin-stimulated glucose transport activity in skeletal muscle in rats fed a high-fat diet.
Male Wistar rats were placed on a high-fat diet (n=24) or standard chow diet (Chow, n=12) for 10weeks. Rats fed the high-fat diet were separated into two groups after 4weeks. One group was subjected to ADF for the subsequent 6weeks (HF-ADF, n=12), and the other group was maintained on an ad libitum diet (HF-AL, n=12). After the 10-week dietary intervention, measurements of insulin-stimulated glucose uptake and insulin tolerance test (ITT) were performed.
Whereas the total intra-abdominal fat mass in the HF-AL group was significantly higher than in the Chow and HF-ADF groups, there was no significant difference between the Chow and HF-ADF groups. However, insulin-stimulated glucose uptake in skeletal muscles was significantly lower in both high-fat fed groups than in the Chow group. Muscle GLUT-4 protein content in HF-AL is significantly lower (30%) than in Chow, and further reduction (42%) was observed in the HF-ADF group rats. The HF-ADF and HF-AL group rats had less reduction in glycemia than did the Chow group rats during ITT.
ADF was unable to eliminate high-fat diet-induced muscle insulin resistance, despite a substantial decrease in total intra-abdominal fat mass. This might have resulted from a reduction in GLUT-4 protein in both HF-AL and HF-ADF rats compared to the Chow group.
本研究旨在探讨隔日禁食（ADF）对高脂肪饮食喂养大鼠骨骼肌胰岛素刺激葡萄糖转运活性的影响。
雄性 Wistar 大鼠给予高脂肪饮食（n=24）或标准饲料（Chow，n=12）10 周。高脂肪饮食喂养的大鼠在第 4 周后分为两组。一组在随后的 6 周内进行 ADF（HF-ADF，n=12），另一组继续自由进食（HF-AL，n=12）。经过 10 周的饮食干预，进行胰岛素刺激葡萄糖摄取和胰岛素耐量试验（ITT）的测量。
尽管 HF-AL 组的总腹腔内脂肪量明显高于 Chow 和 HF-ADF 组，但 Chow 和 HF-ADF 组之间没有显著差异。然而，两组高脂肪喂养大鼠的骨骼肌胰岛素刺激葡萄糖摄取均明显低于 Chow 组。HF-AL 大鼠的肌肉 GLUT-4 蛋白含量明显低于 Chow 组（30%），而 HF-ADF 组大鼠的含量进一步降低（42%）。在 ITT 中，HF-ADF 和 HF-AL 组大鼠的血糖降低幅度小于 Chow 组大鼠。
尽管总腹腔内脂肪量明显减少，但 ADF 未能消除高脂肪饮食引起的肌肉胰岛素抵抗。这可能是由于与 Chow 组相比，HF-AL 和 HF-ADF 组大鼠的 GLUT-4 蛋白减少所致。
