Habiby Mehrdad, Ezati Pourya, Soltanian Danial, Rahehagh Ramesh, Hosseini Foruzan
Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Department of Pathology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Heliyon. 2024 Feb 14;10(4):e25708. doi: 10.1016/j.heliyon.2024.e25708. eCollection 2024 Feb 29.
Intermittent fasting (IF), describes a variety of diets that the individual is exposed to intermittent periods of fasting and refeeding. The present study was designed to compare the three most popular intermittent fasting methods in high-fat-diet-induced obese mice.
50 male C57BL/6 mice were divided into Normal Control (CN), High-Fat Control (CH), IF1 (Alternate Day Fasting), IF2 (Time-Restricted Feeding), IF3 (5:2 Diet) diets. In the first phase of the study, the mice were fed ad libitum either with a 54% Calorie high-fat (CH, IF1, IF2, IF3) or standard CHOW (CN) for 8 weeks. Then, in the intervention phase, the IF groups were fasted for four weeks based on their fasting protocol. At the end of the study, the mice fasting blood, liver and fat tissue samples were biochemically and pathologically assessed.
The weight loss during the fasting period in IF1 and IF2 groups was significantly greater than CH. The epididymal fat pad weight was significantly lower in IF2 and IF3 compared to CH. The serum Triglyceride was significantly greater in CH than in the CN group. The tissue injury scores of the high-fat-diet groups were significantly greater than CN. Also, the tissue injury score was greater in IF1 group compared to the high-fat control group.
All of the fasting protocols can prompt acceptable energy restriction and the ADF and TRF protocols can significantly cause weight loss. Also, the TRF and 5:2 Diets can lower the visceral adiposity. However further human studies focusing on dietary adherence seem necessary.
间歇性禁食（IF）描述的是个体经历间歇性禁食和重新进食的多种饮食方式。本研究旨在比较高脂饮食诱导的肥胖小鼠中三种最流行的间歇性禁食方法。
将50只雄性C57BL/6小鼠分为正常对照组（CN）、高脂对照组（CH）、IF1（隔日禁食）、IF2（限时进食）、IF3（5:2饮食）组。在研究的第一阶段，小鼠随意进食54%卡路里的高脂饮食（CH、IF1、IF2、IF3）或标准饲料（CN），持续8周。然后，在干预阶段，IF组根据其禁食方案禁食四周。在研究结束时，对小鼠的空腹血液、肝脏和脂肪组织样本进行生化和病理评估。
IF1组和IF2组在禁食期间的体重减轻明显大于CH组。与CH组相比，IF2组和IF3组的附睾脂肪垫重量明显更低。CH组的血清甘油三酯明显高于CN组。高脂饮食组的组织损伤评分明显高于CN组。此外，IF1组的组织损伤评分高于高脂对照组。
所有禁食方案都能促使能量摄入得到可接受的限制，隔日禁食（ADF）和限时进食（TRF）方案能显著导致体重减轻。此外，限时进食和5:2饮食可以降低内脏脂肪含量。然而，似乎有必要进一步开展针对饮食依从性的人体研究。
