Boehnke M
Department of Biostatics, School of Public Health, University of Michigan, Ann Arbor 48109.
Am J Hum Genet. 1990 Aug;47(2):218-27.
Sample-size guidelines for linkage studies of quantitative traits partially determined by a dominant major locus are needed to provide a rough estimate of the amount of pedigree material that should be sampled to map the loci that influence such traits. After pedigrees are sampled, a specific power calculation can be carried out to evaluate the linkage information provided by the sampled pedigrees. Using computer simulation, I provide sample-size guidelines for linkage studies by the method of lod scores of quantitative traits partially determined by a dominant major locus. I consider the effects of a trait model, marker characteristics, and sampling strategy, with particular attention to sampling strategy because it is the one factor which the investigator can fully control. My results suggest that linkage studies of quantitative traits are practical, particularly if the investigator chooses an efficient sampling design and an efficient strategy to select pedigrees for linkage analysis.
对于部分由显性主基因座决定的数量性状进行连锁研究时，需要样本量指导原则，以便对为定位影响此类性状的基因座而应采集的系谱材料数量提供一个大致估计。在采集系谱后，可以进行特定的功效计算，以评估所采集系谱提供的连锁信息。通过计算机模拟，我针对部分由显性主基因座决定的数量性状，采用对数优势分数法给出了连锁研究的样本量指导原则。我考虑了性状模型、标记特征和抽样策略的影响，特别关注抽样策略，因为它是研究者能够完全控制的一个因素。我的结果表明，数量性状的连锁研究是可行的，尤其是当研究者选择高效的抽样设计和有效的策略来选择用于连锁分析的系谱时。
