Karawya M S, Hifnawy M S, Dahawi H S
J Assoc Off Anal Chem. 1975 Jan;58(1):85-7.
Two colorimetric methods are described for the estimation of strychnine and brucine in nux vomica. The first is a modification of the Karawya and Ghourab method for the determination of strychnine, in which the sensitivity of the color is increased by changing certain conditions of the method. The second was developed for the determination of brucine and is based on measuring the intensity of the violet color produced by treating brucine with nitric acid and methanolic stannous chloride. In the presence of large amounts of strychnine, brucine is isolated prior to colorimetric analysis by a quantitative thin layer chromatographic technique.
本文描述了两种比色法，用于测定马钱子中士的宁和马钱子碱的含量。第一种方法是对卡拉维亚（Karawya）和古拉卜（Ghourab）测定士的宁方法的改进，通过改变该方法的某些条件提高了显色的灵敏度。第二种方法是为测定马钱子碱而开发的，其基于测量马钱子碱与硝酸和甲醇氯化亚锡反应产生的紫色强度。在存在大量士的宁的情况下，通过定量薄层色谱技术在比色分析前分离马钱子碱。
