Pierangelo Angelo, Nazac André, Benali Abdelali, Validire Pierre, Cohen Henri, Novikova Tatiana, Ibrahim Bicher Haj, Manhas Sandeep, Fallet Clément, Antonelli Maria-Rosaria, Martino Antonello-De
Laboratoire de Physique des Interfaces et des Couches Minces (LPICM), Ecole polytechnique, CNRS, 91128 Palaiseau, France.
Opt Express. 2013 Jun 17;21(12):14120-30. doi: 10.1364/OE.21.014120.
We present a preliminary investigation of macroscopic polarimetric imaging of uterine cervix. Orthogonal state contrast (OSC) images of healthy and anomalous cervices have been taken in vivo at 550 nm. Four ex vivo cervix samples have been studied in full Muller polarimetry, at 550 nm and 700 nm, and characterized in detail by standard pathology. One sample was totally healthy, another one carried CIN lesions at very early stage (CIN1) in its visible exocervical region, while for the other two samples more advanced (CIN3) lesions were present, together with visible glandular epithelium (ectropion). Significant birefringence has been observed in the healthy regions of all six samples, both in vivo and ex vivo. Standard treatments of the Mueller images of the ex vivo samples allowed to quantify both retardation and depolarization. Retardation reached 60° in healthy regions, and disappeared in the anomalous regions of the other three ex vivo samples. The depolarization power was largest in healthy regions, and lower in CINs and ectropion. Possible origins of the observed effects are briefly discussed.
我们展示了一项关于子宫颈宏观偏振成像的初步研究。在550纳米波长下对健康和异常子宫颈进行了体内正交状态对比（OSC）成像。对四个离体子宫颈样本进行了全穆勒偏振测量，测量波长为550纳米和700纳米，并通过标准病理学进行了详细表征。一个样本完全健康，另一个在其可见的子宫颈外区域处于非常早期阶段（CIN1）携带CIN病变，而另外两个样本存在更高级别的（CIN3）病变，同时伴有可见的腺上皮（外翻）。在所有六个样本的健康区域，无论体内还是离体，均观察到了显著的双折射现象。对离体样本的穆勒图像进行标准处理后，可以对延迟和去极化进行量化。健康区域的延迟达到60°，而在其他三个离体样本的异常区域则消失。去极化能力在健康区域最大，在CIN病变区域和外翻区域较低。我们简要讨论了观察到的这些效应的可能来源。
