Kang Pilsoo, Madera Milan, Alley William R, Goldman Radoslav, Mechref Yehia, Novotny Milos V
National Center for Glycomics and Glycoproteomics ; Department of Chemistry, Bloomington, IN, USA, 47405.
Int J Mass Spectrom. 2011 Aug 15;305(2-3):185-198. doi: 10.1016/j.ijms.2010.11.007.
Hepatocellular cancer is a serious human disease with an unfortunately low survival rate. It further poses a significant epidemic threat to our society through its viral vectors associated with cirrhosis conditions preceding the cancer. A search for biomarkers of these diseases enlists analytical glycobiology, in general, and quantitative biomolecular mass spectrometry (MS), in particular, as valuable approaches to cancer research. The recent advances in quantitative glycan permethylation prior to MALDI-MS oligosaccharide profiling has enabled us to compare the glycan quantitative proportions in the small serum samples of cancer and cirrhotic patients against control individuals. We were further able to fractionate the major serum proteins from the minor components and compare statistically their differential glycosylation, elucidating some causes of quantitatively unusual glycosylation events. Numerous glycan structures were tentatively identified and connected with the origin proteins, with a particular emphasis on sialylated and fucosylated glycans.
肝细胞癌是一种严重的人类疾病，生存率低得令人遗憾。它还通过与癌症之前的肝硬化状况相关的病毒载体，对我们的社会构成重大的流行威胁。一般来说，寻找这些疾病的生物标志物需要借助分析糖生物学，特别是定量生物分子质谱（MS），作为癌症研究的重要方法。基质辅助激光解吸电离质谱（MALDI-MS）寡糖谱分析之前的定量聚糖全甲基化技术的最新进展，使我们能够比较癌症患者和肝硬化患者的少量血清样本与对照个体之间聚糖的定量比例。我们还能够从小分子成分中分离出主要的血清蛋白，并对它们的差异糖基化进行统计学比较，阐明了一些定量异常糖基化事件的原因。初步鉴定了许多聚糖结构，并将其与起源蛋白联系起来，特别关注唾液酸化和岩藻糖化聚糖。
