高灵敏度分析糖生物学：当前方法和方向。
Analytical glycobiology at high sensitivity: current approaches and directions.
机构信息
Department of Chemistry, Indiana University, Bloomington, IN, USA.
出版信息
Glycoconj J. 2013 Feb;30(2):89-117. doi: 10.1007/s10719-012-9444-8. Epub 2012 Sep 4.
Abstract
This review summarizes the analytical advances made during the last several years in the structural and quantitative determinations of glycoproteins in complex biological mixtures. The main analytical techniques used in the fields of glycomics and glycoproteomics involve different modes of mass spectrometry and their combinations with capillary separation methods such as microcolumn liquid chromatography and capillary electrophoresis. The need for high-sensitivity measurements have been emphasized in the oligosaccharide profiling used in the field of biomarker discovery through MALDI mass spectrometry. High-sensitivity profiling of both glycans and glycopeptides from biological fluids and tissue extracts has been aided significantly through lectin preconcentration and the uses of affinity chromatography.
摘要
本文综述了近年来在复杂生物混合物中糖蛋白的结构和定量测定方面取得的分析进展。糖组学和糖蛋白质组学领域中使用的主要分析技术涉及不同模式的质谱及其与毛细管分离方法（如微柱液相色谱和毛细管电泳）的组合。在通过 MALDI 质谱进行生物标志物发现的寡糖分析中，强调了高灵敏度测量的必要性。通过凝集素预浓缩和亲和层析的应用，大大促进了生物体液和组织提取物中聚糖和糖肽的高灵敏度分析。
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