Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.
Asian Pac J Trop Med. 2013 Aug;6(8):609-15. doi: 10.1016/S1995-7645(13)60105-4.
To evaluate in vivo antioxidant and hepatoprotective activities of the methanolic extract of the root of Cassia singueana in rats following acute and chronic carbon tetrachloride intoxication.
Malondialdehyde (MDA), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and bilirubin as indices of liver damage and lipid peroxidation were detected in rats after intraperitoneal administration of extract (5 mg/kg).
The liver, kidney and heart showed significant reduction (P<0.05) in the levels of MDA from (0.18±0.04), (0.23±0.07) and (0.26±0.10) nmol/mg respectively in the CCl4 control to (0.15±0.03), (0.17±0.04) and (0.17±0.07) nmol/mg protein in groups pre-treated with the extract for three days at 5 mg/kg). Similarly, compared to the CCl4 control, significant reduction (P<0.05) in serum AST, ALT and bilirubin as well as in level of total cholesterol and MDA with concomitant increase in HDL cholesterol, superoxide dismutase and catalase levels when CCl4-intoxicated rats were treated with Cassia singueana root extract for two weeks.
These results suggest that methanolic extract of Cassia singueana contain potent antioxidant compounds that can offer significant protection against hepatic and oxidative injuries.
评价在体内抗氧化和保肝活性的甲醇提取物的根苦参在大鼠急性和慢性四氯化碳中毒。
丙二醛（MDA），天门冬氨酸氨基转移酶（AST），丙氨酸氨基转移酶（ALT）和胆红素作为肝损伤和脂质过氧化的指标在大鼠腹腔内给药后提取（5 毫克/公斤）。
肝、肾和心脏显著减少（P<0.05）MDA的水平分别为（0.18±0.04），（0.23±0.07）和（0.26±0.10）nmol/mg 在 CCl4 对照组到（0.15±0.03），（0.17±0.04）和（0.17±0.07）nmol/mg 在预处理组蛋白与提取物 3 天 5 毫克/公斤）。同样，与 CCl4 对照组相比，显著减少（P<0.05）血清 AST、ALT 和胆红素以及总胆固醇和 MDA 的水平与高密度脂蛋白胆固醇、超氧化物歧化酶和过氧化氢酶水平升高，当 CCl4 中毒大鼠用苦参根提取物治疗两周。
这些结果表明，苦参甲醇提取物含有有效的抗氧化剂化合物，可以提供显著的保护肝脏和氧化损伤。
