Panahi Kokhdan Esmaeel, Ahmadi Kyomarth, Sadeghi Heibatollah, Sadeghi Hossein, Dadgary Fahemeh, Danaei Nazanin, Aghamaali Mahmoud Reza
a Department of Biology, Faculty of Science , Guilan University , Rasht , Iran.
b Biochemistry Department, Faculty of Medicine , AJA University of Medical Sciences , Tehran , Iran.
Pharm Biol. 2017 Dec;55(1):1389-1393. doi: 10.1080/13880209.2017.1302484.
Stachys pilifera Benth (Lamiaceae) has long been used to treat infectious diseases, respiratory and rheumatoid disorders in Iranian folk medicine. Antitumor and antioxidant activity of the plant have been reported.
The study was designed to assess the hepatoprotective activity of ethanol extract of Stachys pilifera in carbon tetrachloride (CCl)-induced hepatotoxicity in rats.
The rats were randomly divided into six equal groups (n = 7). Group I was treated with normal saline; Group II received CCl (1 mL/kg. i.p., twice a week) for 60 consecutive days; Groups III, IV and V were given CCl plus Stachys pilifera (100, 200 and 400 mg/kg/d,p.o.); Group VI received the extract (400 mg/kg/d, p.o.). Histopathological analysis and measurement of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), malondialdehyde (MDA), total protein (TP) and albumin (ALB) were performed.
CCl caused a significant increase in the serum levels of AST, ALT, ALP and MDA as well as decreased ALB, and TP serum levels (p < 0.001). The extract (200 and 400 mg/kg/d) significantly normalized the CCl-elevated levels of ALT, AST, ALP and MDA (p < 0.001). The extract (200 and 400 mg/kg/d) also increased the serum levels of TP compared to CCl group (p< 0.01). The extract (200 and 400 mg/kg/d) also decreased the histological injuries (inflammation and fatty degeneration) by CCl.
The results revealed that the Stachys pilifera extract could provide considerable protection against CCl hepatotoxicity in rats that may be related to its antioxidant properties.
伊朗民间医学长期以来一直使用绵毛水苏（唇形科）治疗传染病、呼吸系统疾病和类风湿性疾病。该植物的抗肿瘤和抗氧化活性已有报道。
本研究旨在评估绵毛水苏乙醇提取物对四氯化碳（CCl）诱导的大鼠肝毒性的肝保护活性。
将大鼠随机分为六个相等的组（n = 7）。第一组用生理盐水处理；第二组连续60天接受CCl（1 mL/kg，腹腔注射，每周两次）；第三组、第四组和第五组给予CCl加绵毛水苏（100、200和400 mg/kg/d，口服）；第六组接受提取物（400 mg/kg/d，口服）。进行组织病理学分析以及血清天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）、丙二醛（MDA）、总蛋白（TP）和白蛋白（ALB）的测定。
CCl导致血清AST、ALT、ALP和MDA水平显著升高，同时ALB和TP血清水平降低（p < 0.001）。提取物（200和400 mg/kg/d）显著使CCl升高的ALT、AST、ALP和MDA水平恢复正常（p < 0.001）。与CCl组相比，提取物（200和400 mg/kg/d）还使血清TP水平升高（p < 0.01）。提取物（200和400 mg/kg/d）还减轻了CCl引起的组织学损伤（炎症和脂肪变性）。
结果表明，绵毛水苏提取物可以为大鼠提供对CCl肝毒性的显著保护，这可能与其抗氧化特性有关。
