Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
Mar Pollut Bull. 2013 Aug 15;73(1):300-5. doi: 10.1016/j.marpolbul.2013.05.002. Epub 2013 Jun 20.
Petroleum products spill and leakage have become two major environmental challenges in Iran. Sampling was performed in the petroleum reservoir waste water of Tehran and Kerman Provinces of Iran. Alkane degrading bacteria were isolated by enrichment in a Bushnel-Hass medium, with hexadecane as sole source of carbon and energy. The isolated strains were identified by amplification of 16S rDNA gene and sequencing. Specific primers were used for identification of alkane hydroxylase gene. Fifteen alkane degrading bacteria were isolated and 8 strains were selected as powerful degradative bacteria. These 8 strains relate to Rhodococcus jostii, Stenotrophomonas maltophilia, Achromobacter piechaudii, Tsukamurella tyrosinosolvens, Pseudomonas fluorescens, Rhodococcus erythropolis, Stenotrophomonas maltophilia, Pseudomonas aeruginosa genera. The optimum concentration of hexadecane that allowed high growth was 2.5%. Gas chromatography results show that all strains can degrade approximately half of hexadecane in one week of incubation. All of the strains have alkane hydroxylase gene which are important for biodegradation. As a result, this study indicates that there is a high diversity of degradative bacteria in petroleum reservoir waste water in Iran.
石油产品的泄漏和溢漏已成为伊朗面临的两大环境挑战。本研究在伊朗德黑兰省和克尔曼省的石油储存废水中进行采样。通过在 Bushnel-Hass 培养基中进行富集，以十六烷作为唯一的碳源和能源，分离出烷烃降解菌。通过扩增 16S rDNA 基因和测序对分离株进行鉴定。使用特定引物鉴定烷烃羟化酶基因。共分离出 15 株烷烃降解菌，并选择 8 株作为高效降解菌。这 8 株菌分别属于琼氏红球菌、寡养单胞菌、食烷假单胞菌、游动放线菌、荧光假单胞菌、红景天红球菌、寡养单胞菌和铜绿假单胞菌。允许高生长的十六烷最佳浓度为 2.5%。气相色谱结果表明，所有菌株在孵育一周内均可降解约一半的十六烷。所有菌株都具有烷烃羟化酶基因，这对于生物降解非常重要。因此，本研究表明伊朗石油储存废水中存在高度多样化的降解菌。
