State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
J Genet Genomics. 2013 Jun 20;40(6):271-9. doi: 10.1016/j.jgg.2013.03.009. Epub 2013 Mar 26.
Precise genome modification with engineered nucleases is a powerful tool for studying basic biology and applied biotechnology. Transcription activator-like effector nucleases (TALENs), consisting of an engineered specific (TALE) DNA binding domain and a Fok I cleavage domain, are newly developed versatile reagents for genome engineering in different organisms. Because of the simplicity of the DNA recognition code and their modular assembly, TALENs can act as customizable molecular DNA scissors inducing double-strand breaks (DSBs) at given genomic location. Thus, they provide a valuable approach to targeted genome modifications such as mutations, insertions, replacements or chromosome rearrangements. In this article, we review the development of TALENs, and summarize the principles and tools for TALEN-mediated gene targeting in plant cells, as well as current and potential strategies for use in plant research and crop improvement.
精确的基因组修饰与工程化的核酸酶是研究基础生物学和应用生物技术的有力工具。转录激活子样效应物核酸酶（TALENs）由一个工程化的特异性（TALE）DNA 结合域和一个 Fok I 切割域组成，是在不同生物中进行基因组工程的新型多功能试剂。由于 DNA 识别密码的简单性及其模块化组装，TALENs 可以作为可定制的分子 DNA 剪刀，在给定的基因组位置诱导双链断裂（DSB）。因此，它们为靶向基因组修饰提供了一种有价值的方法，如突变、插入、替换或染色体重排。在本文中，我们回顾了 TALENs 的发展，并总结了 TALEN 介导的植物细胞基因靶向的原理和工具，以及在植物研究和作物改良中的当前和潜在策略。
