Molecular characterization and expression of cyclic nucleotide gated ion channels 19 and 20 in for their potential role in salt stress.

Cyclic nucleotide gated ion channels (CNGCs) in plants have very important role in signaling and development. The study reports role of CNGC19 and CNGC20 in salt stress in genome wide analysis showed that and are related to salt stress with maximum expression after 6 h in . The position of inserted T-DNA was determined ( through TAIL-PCR for activation tagged mutants of and under salt stress. The expression of and after cloning under 35S promoter of expression vectors pBCH1 and pEarleyGate100 was determined in by real-time PCR analysis. Genome wide analysis showed that AtCNGC11 had lowest and AtCNGC20 highest molecular weight as well as number of amino acid residues. expression of and was enhanced through T-DNA insertion and 35S promoter in over-expressed plants under high salt concentration. was activated twice in control and about five times under 150 mM NaCl stress level, and expression value was also higher than . Phenotypically, over-expressed plants and calli were healthier while knock-out plants and calli showed retarded growth under salinity stress. The study provides new insight for the role of AtCNGC19 and AtCNGC20 under salt stress regulation in and will be helpful for improvement of crop plants for salt stress to combat food shortage and security.