State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.
Food Chem. 2013 Nov 15;141(2):754-61. doi: 10.1016/j.foodchem.2013.03.044. Epub 2013 Mar 21.
The B group vitamin known as folates is essential for a variety of physiological processes and plays an important role in the prevention of neural tube defects. However, it decomposes when exposed to UV light. In this study, the response of the synthetic form of folates known as folic acid to UV irradiation in the presence of β-lactoglobulin (β-LG), bovine serum albumin (BSA) and α-lactalbumin (α-LA) was investigated using circular dichroism, absorbance and fluorescence spectroscopy. Photodecomposition of folic acid was delayed in the presence of the proteins, which ranked in the order β-LG>BSA>α-LA in terms of effectiveness. Protein unfolding or decomposition occurred at the same time, due to interaction with folic acid photodecomposition products. The results suggest potential uses of ligand-binding proteins as carriers of water-soluble active compounds for nutraceutical applications.
B 族维生素叶酸对各种生理过程至关重要，对预防神经管缺陷起着重要作用。然而，它在暴露于紫外线下时会分解。在这项研究中，使用圆二色性、吸光度和荧光光谱法研究了在β-乳球蛋白（β-LG）、牛血清白蛋白（BSA）和α-乳白蛋白（α-LA）存在下，合成叶酸形式的叶酸对紫外线照射的反应。在蛋白质存在下，叶酸的光解被延迟，按效果排列，蛋白质的顺序为β-LG>BSA>α-LA。由于与叶酸光解产物的相互作用，蛋白质的展开或分解同时发生。结果表明，配体结合蛋白作为水可溶性活性化合物的载体在营养保健品应用中有潜在用途。
