Laboratory of Food & Nutritional Sciences, Faculty of Nutrition, Kobe Gakuin University, Nishi-ku, Kobe, Hyogo 651-2180, Japan.
Food Chem. 2013 Nov 15;141(2):1000-7. doi: 10.1016/j.foodchem.2013.04.048. Epub 2013 Apr 24.
During the screening of selective DNA polymerase (pol) inhibitors, we isolated cycloartenyl trans-ferulate (CAF), which is a major component of γ-oryzanol, which is a byproduct formed during the production of Japanese rice wine "sake". CAF selectively inhibited the activity of mammalian A, B, and X pol families, but Y family pols were not affected. CAF did not influence the activities of plant or prokaryotic pols, nor the activity of other DNA metabolic enzymes tested. Individual chemical components of CAF, including cycloartenol (CA) and ferulic acid (FA), did not inhibit pol enzyme activities. CAF suppressed TPA (12-O-tetradecanoylphorbol-13-acetate)-induced inflammation in the mouse ear, but CA and FA did not. The ability to inhibit mammalian pol enzymes in vitro was positively correlated with their propensity to suppress inflammation in vivo. These results suggest that this byproduct formed during the sake-brewing process is useful as an anti-inflammatory agent.
在筛选选择性 DNA 聚合酶 (pol) 抑制剂的过程中，我们分离出环阿屯基反式-咖啡酸酯（CAF），它是 γ-谷维素的主要成分，γ-谷维素是日本米酒“清酒”生产过程中的副产物。CAF 选择性抑制哺乳动物 A、B 和 X pol 家族的活性，但 Y 家族 pol 不受影响。CAF 不影响植物或原核 pol 的活性，也不影响测试的其他 DNA 代谢酶的活性。CAF 的单个化学成分，包括环阿屯醇（CA）和阿魏酸（FA），不抑制 pol 酶活性。CAF 抑制 TPA（12-O-十四烷酰佛波醇-13-乙酸酯）诱导的小鼠耳炎症，而 CA 和 FA 则没有。体外抑制哺乳动物 pol 酶的能力与其在体内抑制炎症的倾向呈正相关。这些结果表明，这种在清酒酿造过程中形成的副产物可用作抗炎剂。
