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测定石榴汁中的氨基酸并对其与苹果汁混合进行指纹图谱分析。

Determination of amino acids in pomegranate juices and fingerprint for adulteration with apple juices.

机构信息

Istanbul Technical University, Department of Chemistry, Maslak, Istanbul, Turkey.

出版信息

Food Chem. 2013 Nov 15;141(2):1187-91. doi: 10.1016/j.foodchem.2013.04.017. Epub 2013 Apr 17.

DOI:10.1016/j.foodchem.2013.04.017
PMID:23790902
Abstract

A new chiral micellar electrokinetic chromatography-laser induced fluorescence (MEKC-LIF) method was developed using sodium dodecylbenzene sulphonate (SDBS) as surfactant for the determination of chiral amino acids in pomegranate juices. The use of SDBS as the micellar medium enhanced the fluorescence intensities of amino acids derivatised with fluorescein isothiocyanate (FITC). The amino acid profile of pomegranate juices was compared to apple amino acids and l-Asn was proposed as a marker for the adulteration of pomegranate juices with apple juices.

摘要

建立了一种以十二烷基苯磺酸钠(SDBS)为胶束增敏剂的手性胶束电动色谱-激光诱导荧光(MEKC-LIF)检测方法,用于测定石榴汁中的手性氨基酸。SDBS 作为胶束介质增强了氨基酸与异硫氰酸荧光素(FITC)衍生化后的荧光强度。比较了石榴汁和苹果汁中的氨基酸组成,提出 l-Asn 可作为掺假苹果汁的标志物。

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