Laboratory of Protein Engineering and Bioactive Molecules (LIP-MB), National Institute of Applied Sciences and Technology (INSAT), University of Carthage, Tunis, Tunisia.
Food Chem. 2013 Nov 15;141(2):1398-405. doi: 10.1016/j.foodchem.2013.04.008. Epub 2013 Apr 18.
The γ-irradiation effects on polyphenolic content and antioxidant capacity of parathion-pretreated leaves of Salvia officinalis plant were investigated. The analysis of phenolic extracts of sage without parathion showed that irradiation decreased polyphenolic content significantly (p<0.05) by 30% and 45% at 2 and 4kGy, respectively, compared to non-irradiated samples. The same trend was observed for the Trolox equivalent antioxidant capacity (TEAC), as assessed by the anionic DPPH and cationic ABTS radical-scavenging assays. The antioxidant potential decreased significantly (p<0.01) at 2 and 4kGy, by 11-20% and 40-44%, respectively. The results obtained with a pure chlorogenic acid solution confirmed the degradation of phenols; however, its TEAC was significantly (p<0.01) increased following irradiation. Degradation products of parathion formed by irradiation seem to protect against a decline of antioxidant capacity and reduce polyphenolic loss. Ionising radiation was found to be useful in breaking down pesticide residues without inducing significant losses in polyphenols.
研究了γ辐照对预先用对硫磷处理的鼠尾草叶片中多酚含量和抗氧化能力的影响。分析未经对硫磷处理的鼠尾草酚类提取物的结果表明，与未辐照样品相比，辐照分别在 2 和 4kGy 时使多酚含量显著降低（p<0.05），分别为 30%和 45%。用阴离子 DPPH 和阳离子 ABTS 自由基清除试验评估的 Trolox 等效抗氧化能力（TEAC）也呈现相同的趋势。在 2 和 4kGy 时，抗氧化能力显著降低（p<0.01），分别降低了 11-20%和 40-44%。用纯绿原酸溶液获得的结果证实了酚类物质的降解；然而，其 TEAC 在辐照后显著增加（p<0.01）。辐照形成的对硫磷降解产物似乎可以防止抗氧化能力下降，并减少多酚损失。发现电离辐射可用于分解农药残留，而不会导致多酚的显著损失。
