高效液相色谱柱后荧光衍生法同时测定大鼠血清中2-脱氧-D-葡萄糖和D-葡萄糖
Simultaneous determination of 2-deoxy-D-glucose and D-glucose in rat serum by high-performance liquid chromatography with post-column fluorescence derivatization.
作者信息
Umegae Y, Nohta H, Ohkura Y
机构信息
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
出版信息
Chem Pharm Bull (Tokyo). 1990 Apr;38(4):963-5. doi: 10.1248/cpb.38.963.
A simple high-performance liquid chromatographic method for the determination of 2-deoxy-D-glucose and D-glucose in rat serum is described; this method is based on a post-column fluorescence derivatization. The sugars are automatically converted into fluorescent derivatives by reaction with meso-1,2-bis(4-methoxyphenyl)ethylenediamine in an alkaline medium after their separation on a strong anion exchanger column (TSK gel Sugar AXG). The detection limits (S/N = 3) for 2-deoxy-D-glucose and D-glucose in rat serum are 0.52 and 0.56 nmol/ml, respectively.
描述了一种用于测定大鼠血清中2-脱氧-D-葡萄糖和D-葡萄糖的简单高效液相色谱方法；该方法基于柱后荧光衍生化。糖在强阴离子交换柱（TSK凝胶糖AXG）上分离后，在碱性介质中与内消旋-1,2-双（4-甲氧基苯基）乙二胺反应，自动转化为荧光衍生物。大鼠血清中2-脱氧-D-葡萄糖和D-葡萄糖的检测限（S/N = 3）分别为0.52和0.56 nmol/ml。
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