Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China.
Appl Microbiol Biotechnol. 2013 Aug;97(15):7061-71. doi: 10.1007/s00253-013-5027-2. Epub 2013 Jun 23.
Although successful production of fatty alcohols in metabolically engineered Escherichia coli with heterologous expression of fatty acyl-CoA reductase has been reported, low biosynthetic efficiency is still a hurdle to be overcome. In this study, we examined the characteristics of two fatty acyl-CoA reductases encoded by Maqu_2220 and Maqu_2507 genes from Marinobacter aquaeolei VT8 on fatty alcohol production in E. coli. Fatty alcohols with diversified carbon chain length were obtained by co-expressing Maqu_2220 with different carbon chain length-specific acyl-ACP thioesterases. Both fatty acyl-CoA reductases displayed broad substrate specificities for C12-C18 fatty acyl chains in vivo. The optimized mutant strain of E. coli carrying the modified tesA gene and fadD gene from E. coli and Maqu_2220 gene from Marinobacter aquaeolei VT8 produced fatty alcohols at a remarkable level of 1.725 g/L under the fermentation condition.
尽管已经有报道称，通过异源表达脂肪酸酰基辅酶 A 还原酶，在代谢工程大肠杆菌中成功生产了脂肪醇，但生物合成效率低仍是一个亟待克服的障碍。在本研究中，我们研究了来自海栖热袍菌 VT8 的 Maqu_2220 和 Maqu_2507 基因编码的两种脂肪酸酰基辅酶 A 还原酶在大肠杆菌中生产脂肪醇的特性。通过共表达 Maqu_2220 与不同碳链长度特异性酰基-ACP 硫酯酶，获得了具有多样化碳链长度的脂肪醇。两种脂肪酸酰基辅酶 A 还原酶在体内均显示出对 C12-C18 脂肪酸链的广泛底物特异性。在发酵条件下，携带来自大肠杆菌的修饰后的 tesA 基因和 fadD 基因以及来自海栖热袍菌 VT8 的 Maqu_2220 基因的优化突变大肠杆菌菌株可生产 1.725g/L 的脂肪醇，产量显著提高。
