Fillet Sandy, Gibert Jordi, Suárez Beatriz, Lara Armando, Ronchel Carmen, Adrio José L
Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain.
J Ind Microbiol Biotechnol. 2015 Nov;42(11):1463-72. doi: 10.1007/s10295-015-1674-x. Epub 2015 Aug 29.
We have engineered Rhodosporidium toruloides to produce fatty alcohols by expressing a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8. Production of fatty alcohols in flasks was achieved in different fermentation media at titers ranging from 0.2 to 2 g/L. In many of the conditions tested, more than 80 % of fatty alcohols were secreted into the cultivation broth. Through fed-batch fermentation in 7 L bioreactors, over 8 g/L of C(16)-C(18) fatty alcohols were produced using sucrose as the substrate. This is the highest titer ever reported on microbial production of fatty alcohols to date.
我们通过表达来自嗜油海洋杆菌VT8的脂肪酰辅酶A还原酶，对圆红冬孢酵母进行了工程改造，以生产脂肪醇。在摇瓶中，脂肪醇在不同发酵培养基中的产量为0.2至2 g/L。在许多测试条件下，超过80%的脂肪醇分泌到了培养液中。通过在7 L生物反应器中进行补料分批发酵，以蔗糖为底物生产出了超过8 g/L的C(16)-C(18)脂肪醇。这是迄今为止微生物生产脂肪醇所报道的最高产量。
