Bigham-Sadegh Amin, Torkestani Haleh Sadat, Sharifi Siavash, Shirian Sadegh
Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Department of Veterinary Pathology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Heliyon. 2020 Jul 30;6(7):e04547. doi: 10.1016/j.heliyon.2020.e04547. eCollection 2020 Jul.
Bone grafts have been used to enhance bone fracture healing in orthopedic surgery. Bone grafts enhance bone healing either by mechanical support or acting as a scaffold for bone formation. Fresh autograft is the most effective biomaterial because it is histocompatible with less complication about transmissible disease. Hydroxyapatite is a well-established material for bone repair and very comparable to natural apatite providing a strong biomechanical interlock with host tissue. Royal jelly is the principal food for the honeybee queen. This biomaterial has been demonstrated to have several pharmacological activities, such as antiallergic, antitumor and anti-inflammatory effects.
This study was design to evaluate the effect of concurrent using of Royal jelly with hydroxyapatite on bone healing in rabbit model.
15 adult rabbits weighting approximately 2 kg had been used. They were divided into three groups randomly. In first group (N = 5) mid radius bone defect created and left empty. The second group (N = 5) filled with hydroxy apatite alone and the last group (N = 5) filled with royal jelly and hydroxy apatite combination. Radiological evaluation performed on days14th, 28th and 42nd after operation. Histopathological evaluation was done on 56 postoperative day.
Radiological evaluation showed significant superior bone healing in hydroxyapatite and hydroxyapatite-Royal jelly groups in comparison to control group. Control group was the inferior group between three groups. There were not any significant differences between three groups in histopathological group.
In conclusion our study showed the best results with using the hydroxyl apatite and Royal-jelly group because they provide not only scaffold for bone healing but also do, they provide some osteoinduction materials for bone healing.
骨移植已被用于骨科手术中促进骨折愈合。骨移植通过机械支撑或作为骨形成的支架来促进骨愈合。新鲜自体骨是最有效的生物材料，因为它具有组织相容性，且传染病并发症较少。羟基磷灰石是一种成熟的骨修复材料，与天然磷灰石非常相似，能与宿主组织形成强大的生物力学互锁。蜂王浆是蜂王的主要食物。这种生物材料已被证明具有多种药理活性，如抗过敏、抗肿瘤和抗炎作用。
本研究旨在评估蜂王浆与羟基磷灰石同时使用对兔模型骨愈合的影响。
使用15只体重约2 kg的成年兔子。将它们随机分为三组。第一组（N = 5）制造桡骨中段骨缺损并保持空缺。第二组（N = 5）仅填充羟基磷灰石，最后一组（N = 5）填充蜂王浆和羟基磷灰石的组合。在术后第14天、28天和42天进行放射学评估。在术后第56天进行组织病理学评估。
放射学评估显示，与对照组相比，羟基磷灰石组和羟基磷灰石 - 蜂王浆组的骨愈合明显更好。对照组是三组中效果最差的组。在组织病理学组中，三组之间没有任何显著差异。
总之，我们的研究表明，使用羟基磷灰石和蜂王浆组的效果最佳，因为它们不仅为骨愈合提供支架，而且还为骨愈合提供一些骨诱导材料。
