Naujokat Hendrik, Lipp Maximilian, Açil Yahya, Wieker Henning, Birkenfeld Falk, Sengebusch Andre, Böhrnsen Florian, Wiltfang Jörg
Department of Oral & Maxillofacial Surgery, University Hospital of Schleswig-Holstein, Campus Kiel, Arnold-Heller-Straße 3, 24105 Kiel, Germany.
Department of Oral & Maxillofacial Surgery, University Hospital of Göttingen, Robert-Koch-Straße 40, 37099 Göttingen, Germany.
Regen Med. 2019 Feb;14(2):127-138. doi: 10.2217/rme-2018-0031. Epub 2019 Feb 15.
Reconstruction of bone defects with autologous grafts has certain disadvantages. The aim of this study is to introduce a new type of living bioreactor for engineering of bone flaps and to evaluate the effect of different barrier membranes.
MATERIALS & METHODS: Scaffolds loaded with bone morphogenetic proteins and bone marrow aspirate wrapped with either a collagen membrane or a periosteal flap were implanted in the greater omentum of miniature pigs.
Both histological and radiographic evaluation showed proven bone formation and increased density after 8 and 16 weeks, with an enhanced effect of the periosteal transplant.
The greater omentum is a suitable bioreactor for bone tissue engineering. Endocultivation is both an innovative and promising approach in regenerative medicine.
使用自体移植物重建骨缺损存在一定缺点。本研究的目的是引入一种新型的用于骨瓣工程的活体生物反应器，并评估不同屏障膜的效果。
将负载骨形态发生蛋白和骨髓抽吸物的支架用胶原膜或骨膜瓣包裹后植入小型猪的大网膜中。
组织学和影像学评估均显示，8周和16周后有骨形成且密度增加，骨膜移植的效果增强。
大网膜是骨组织工程的合适生物反应器。体内培养是再生医学中一种创新且有前景的方法。
