Furuzono Tsutomu, Iwamoto Takashi, Azuma Yoshinao, Okada Masahiro, Sawa Yoshiki
Department of Biomaterial Engineering, School of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa, Wakayama, 649-6493, Japan,
J Artif Organs. 2013 Dec;16(4):451-7. doi: 10.1007/s10047-013-0715-3. Epub 2013 Jun 22.
Carboxyl group-donated silver (Ag) nanoparticles for coating on medical devices were prepared by a two-phase reduction system in situ. AgNO3 was the Ag ion source, tetraoctylammonium bromide [N(C8H17)4Br] the phase-transfer agent, sodium tetrahydroborate (NaBH4) the reducing agent and 10-carboxy-1-decanthiol (C11H22O2S, CDT) the capping agent. The characterizations of the Ag nanoparticles were conducted by diffuse reflectance Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric differential thermal analysis (TG/DTA) and transmission electron microscope. With CDT capped on Ag nanoparticles, we found that the band around 3,100 cm(-1) was attributed to COO-H stretching vibration, two adsorptions at 2,928 and 2,856 cm(-1) to C-H symmetric/anti-symmetric stretching vibration, and at 1,718 cm(-1) to C=O stretching vibration in the FT-IR spectra. The organic components of the carboxylated Ag nanoparticles were 5.8-25.9 wt%, determined by TG/DTA. The particle sizes of the carboxylated Ag nanoparticles were well controlled by the addition of the capping agent, CDT, into the reaction system. The antimicrobial activity of the Ag nanoparticles covered with different contents of CDT against E. coli was evaluated. Smaller-size Ag nanoparticles showed higher antibacterial activity, which depended on a surface area that attached easily to a microorganism cell membrane.
通过原位两相还原体系制备了用于涂覆在医疗器械上的羧基供体银（Ag）纳米颗粒。硝酸银（AgNO₃）为银离子源，四丁基溴化铵[N(C₈H₁₇)₄Br]为相转移剂，硼氢化钠（NaBH₄）为还原剂，10 - 羧基 - 1 - 癸硫醇（C₁₁H₂₂O₂S，CDT）为封端剂。通过漫反射傅里叶变换红外（FT - IR）光谱、热重差热分析（TG/DTA）和透射电子显微镜对银纳米颗粒进行了表征。在银纳米颗粒上包覆CDT后，我们发现FT - IR光谱中3100 cm⁻¹附近的谱带归因于 - COO - H伸缩振动，2928和2856 cm⁻¹处的两个吸收峰归因于C - H对称/反对称伸缩振动，1718 cm⁻¹处的吸收峰归因于C = O伸缩振动。通过TG/DTA测定，羧基化银纳米颗粒的有机成分含量为5.8 - 25.9 wt%。通过向反应体系中添加封端剂CDT，可以很好地控制羧基化银纳米颗粒的粒径。评估了包覆不同含量CDT的银纳米颗粒对大肠杆菌的抗菌活性。较小尺寸的银纳米颗粒显示出更高的抗菌活性，这取决于其易于附着在微生物细胞膜上的表面积。
