Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Int J Nanomedicine. 2011;6:581-90. doi: 10.2147/IJN.S17112. Epub 2011 Mar 25.
Silver nanoparticles (Ag NPs) were synthesized by the chemical reducing method in the external and interlamellar space of montmorillonite (MMT) as a solid support at room temperature. AgNO(3) and NaBH(4) were used as a silver precursor and reducing agent, respectively. The most favorable experimental conditions for synthesizing Ag NPs in the MMT are described in terms of the initial concentration of AgNO(3). The interlamellar space limits changed little (d-spacing = 1.24-1.47 nm); therefore, Ag NPs formed on the MMT suspension with d-average = 4.19-8.53 nm diameter. The Ag/MMT nanocomposites (NCs), formed from AgNO(3)/MMT suspension, were characterizations with different instruments, for example UV-visible, PXRD, TEM, SEM, EDXRF, FT-IR, and ICP-OES analyzer. The antibacterial activity of different sizes of Ag NPs in MMT were investigated against Gram-positive, ie, Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) and Gram-negative bacteria, ie, Escherichia coli, Escherichia coli O157:H7, and Klebsiella pneumoniae, by the disk diffusion method using Mueller-Hinton agar (MHA). The smaller Ag NPs were found to have significantly higher antibacterial activity. These results showed that Ag NPs can be used as effective growth inhibitors in different biological systems, making them applicable to medical applications.
银纳米粒子（Ag NPs）通过化学还原法在蒙脱石（MMT）的外表面和层间空间中合成，作为室温下的固体支撑。AgNO3 和 NaBH4 分别用作银前体和还原剂。描述了在 MMT 中合成 Ag NPs 的最有利的实验条件是 AgNO3 的初始浓度。层间空间的变化很小（d 间距=1.24-1.47nm）；因此，在 MMT 悬浮液中形成的 Ag NPs 的 d 平均直径为 4.19-8.53nm。Ag/MMT 纳米复合材料（NCs）由 AgNO3/MMT 悬浮液形成，并用不同的仪器进行了表征，例如 UV-可见分光光度计、PXRD、TEM、SEM、EDXRF、FT-IR 和 ICP-OES 分析仪。采用 MHA 平板扩散法研究了不同尺寸的 Ag NPs 在 MMT 中的抗菌活性，对抗革兰氏阳性菌，即金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）和革兰氏阴性菌，即大肠杆菌、大肠杆菌 O157：H7 和肺炎克雷伯菌。结果表明，较小的 Ag NPs 具有更高的抗菌活性。这些结果表明，Ag NPs 可以作为不同生物系统中的有效生长抑制剂，使其适用于医疗应用。
