Ahmad Bushra, Khan Muhammad Rashid, Shah Naseer Ali
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Toxicol Ind Health. 2015 Dec;31(12):1243-51. doi: 10.1177/0748233713487245. Epub 2013 Jun 24.
This research work was planned to investigate the antioxidant potential of methanolic crude extract of Oxalis corniculata (OCME) against lung injuries initiated by carbon tetrachloride (CCl4) in rats at histological and biochemical level. A total of 42 female Sprague Dawley rats were randomly distributed in to seven groups and each group comprised of six rats. Experiment was completed in 22 days (10 doses at alternate days). Group I was not treated (control rats), while group II was administered with vehicles (olive oil and dimethyl sulfoxide), groups III, IV, and V were treated with 1 ml kg(-1) body weight (b.w.) of CCl4 (20% in olive oil). Group III received only CCl4, whereas groups IV and V were administered with 100 and 200 mg kg(-1) b.w. of OCME, respectively. Group VI was administered with OCME (200 mg kg(-1) b.w.) alone. Group VII was treated with sylimarin (50 mg kg(-1) b.w.). CCl4 enhanced the lipid peroxidation while reduced the glutathione in lung samples. Activities of antioxidant enzymes, catalase, peroxidase, superoxide dismutase, and glutathione-S-transferase decreased in lung homogenates with CCl4. Treatment of CCl4 induced deleterious changes in the microanatomy of lungs by rupturing the alveolar septa, thickening of alveolar walls, and damaging the cells with subsequent collapse of blood vessels due to the accumulation of degenerated blood cells. OCME, dose dependently, prevented the alterations in these parameters. These results suggest that OCME protected the lungs due to its intrinsic properties by scavenging of free radicals generated by CCl4.
本研究旨在从组织学和生物化学水平研究酢浆草甲醇粗提物（OCME）对四氯化碳（CCl4）诱导的大鼠肺损伤的抗氧化潜力。总共42只雌性Sprague Dawley大鼠被随机分为7组，每组6只。实验在22天内完成（隔天给药10次）。第一组未接受治疗（对照大鼠），第二组给予赋形剂（橄榄油和二甲基亚砜），第三、四、五组用1 ml kg(-1)体重（b.w.）的CCl4（20%溶于橄榄油）处理。第三组仅接受CCl4，而第四组和第五组分别给予100和200 mg kg(-1) b.w.的OCME。第六组单独给予OCME（200 mg kg(-1) b.w.）。第七组用西利马林（50 mg kg(-1) b.w.）治疗。CCl4增强了肺组织样本中的脂质过氧化作用，同时降低了谷胱甘肽水平。CCl4处理后，肺匀浆中抗氧化酶过氧化氢酶、过氧化物酶、超氧化物歧化酶和谷胱甘肽-S-转移酶的活性降低。CCl4处理导致肺部微观解剖结构出现有害变化，表现为肺泡间隔破裂、肺泡壁增厚以及细胞损伤，随后由于变性血细胞的积累导致血管塌陷。OCME剂量依赖性地预防了这些参数的改变。这些结果表明，OCME因其内在特性通过清除CCl4产生的自由基而保护了肺组织。
