Department of Biology, Molecular Biology and Genetics Program, Faculty of Science, Firat University, Elazig, Turkey.
Department of Biology, Faculty of Science, Firat University, Elazig, Turkey.
Mol Biol Rep. 2020 Apr;47(4):2573-2582. doi: 10.1007/s11033-020-05340-7. Epub 2020 Mar 2.
The goal of this study was to determine the protective role of ellagic acid (EA) against CCl-induced muscle injury in rats. In this study, 36 Wistar albino rats (n = 36, 8 weeks old) were used. The rats were divided into 4 groups and 9 rats were included each group. Groups: (i) control Group: standard diet; (ii) EA Group: standard diet + EA group; (iii) CCl group: standard diet + CCl group; (iv) EA + CCl group: standard diet + EA + CCl. The animals were decapitated after 8 weeks, and their muscle tissues were received and investigated. In the muscle tissue, TNF-α, COX-2, Nrf-2, NF-kB, caspase-3 and bcl-2 expression levels were analyzed by the western blotting technique, lipid peroxidation was detected by MDA (malondialdehyde), and catalase and GSH levels were determined by a spectrophotometer. In our findings, in comparison to the CCl group, in the EA + CCl group, the Nrf-2 and caspase-3 protein expression levels, GSH and catalase activities increased, while the NF-kB, bcl-2, TNF-α and COX-2 protein expression levels and MDA levels decreased. These results suggest that EA reduces muscle tissue damage rate in rats and that EA may also be used as a potential drug to protect against muscle tissue damage in the future.
本研究旨在探讨鞣花酸（EA）对 CCl 诱导的大鼠肌肉损伤的保护作用。本研究共使用 36 只 Wistar 白化大鼠（n=36，8 周龄）。大鼠分为 4 组，每组 9 只。各组分别为：（i）对照组：标准饮食；（ii）EA 组：标准饮食+EA 组；（iii）CCl 组：标准饮食+CCl 组；（iv）EA+CCl 组：标准饮食+EA+CCl 组。8 周后，大鼠断头处死，取其肌肉组织进行检测。在肌肉组织中，采用 Western blot 技术分析 TNF-α、COX-2、Nrf-2、NF-kB、caspase-3 和 bcl-2 的表达水平，通过 MDA（丙二醛）检测脂质过氧化，通过分光光度计测定 CAT 和 GSH 水平。研究结果表明，与 CCl 组相比，EA+CCl 组的 Nrf-2 和 caspase-3 蛋白表达水平、GSH 和 CAT 活性升高，而 NF-kB、bcl-2、TNF-α和 COX-2 蛋白表达水平及 MDA 水平降低。这些结果提示 EA 可降低大鼠肌肉组织的损伤率，EA 可能作为一种潜在的药物，未来用于肌肉组织损伤的防护。
