Department of Pathology, Guangdong Provincial Hospital of TCM, Guangzhou University of Chinese Medicine, 111 Dade Road, Guangzhou 510120, China; Guangdong Provincial Academy of Chinese Medical Sciences, 111 Dade Road, Guangzhou 510120, China.
Exp Mol Pathol. 2013 Oct;95(2):144-50. doi: 10.1016/j.yexmp.2013.06.009. Epub 2013 Jun 21.
The aim of the present study was to optimize the pregelatinized starch technique for cell block preparation and apply this approach in cultured cells of all types of growing forms, suspension and adherent. In order to evenly mix the starch powder and the cell suspension, we crafted a special plastic dropper. To prove the effectiveness of this optimized technique we used different cell lines, NCI-H69, NCI-H345, HCT-116, SKBR3 and MDA-MB-231. The morphology features, immunocytochemistry (ICC) and fluorescent/chromogenic in-situ hybridization (FISH/CISH) on the cell block sections were evaluated. The morphology features, the ICC and ISH results of cell block sections prepared by the new method were satisfactory comparing with the results obtained in biopsies, the gold standard test for this kind of analysis. The most attractive advantage of our optimized pregelatinized starch technique is that this new method is based on cell suspensions instead of cell sediment, so with our technique every section will contain cells due to the even distribution of the starch powder and the cells forming a homogeneous cell block. To the authors' knowledge, this is the first description on cell block preparation based on cell suspension.
本研究旨在优化细胞块制备的预糊化淀粉技术，并将其应用于各种生长形态、悬浮和贴壁培养的细胞。为了均匀混合淀粉粉末和细胞悬浮液，我们特制了一种特殊的塑料滴管。为了证明该优化技术的有效性，我们使用了不同的细胞系，包括 NCI-H69、NCI-H345、HCT-116、SKBR3 和 MDA-MB-231。评估了细胞块切片的形态特征、免疫细胞化学（ICC）和荧光/显色原位杂交（FISH/CISH）。与活检（这种分析的金标准测试）相比，新方法制备的细胞块切片的形态特征、ICC 和 ISH 结果令人满意。我们优化的预糊化淀粉技术最吸引人的优势是，这种新方法基于细胞悬浮液，而不是细胞沉淀，因此，使用我们的技术，由于淀粉粉末和细胞均匀分布，形成均匀的细胞块，每个切片都会包含细胞。据作者所知，这是首次描述基于细胞悬浮液的细胞块制备。
