Department of Pathology, Guangdong Provincial Hospital of TCM, Guangzhou University of Chinese Medicine, 111 Dade Road, Guangzhou, 510120, China.
Cell Tissue Res. 2012 Nov;350(2):395-400. doi: 10.1007/s00441-012-1467-8. Epub 2012 Aug 16.
Cell block sections serve as an important diagnostic annex for cytological smears, liquid-based SurePath cytology and the Liquid-based Thin-prep Cytology Test (TCT). A variety of methods for the preparation of cell blocks are described in the literature and the techniques in cell blocks are in continuous improvement. A new technique for making cell blocks was introduced in the present study. We first used pregelatinized starch as the frame for the cell block, which is a really simple and economic method, because it can be carried out at room temperature without additional special instruments. We have performed hematoxylin and eosin (HE) staining, immunohistochemistry analysis and fluorescence in situ hybridization (FISH) in the cell block sections in 122 cytological specimens. The results demonstrated in this article show that pregelatinized starch is a useful frame for cell blocks. The pregelatinized starch can effectively collect even a few cells with powerful adhesiveness. Therefore, this new technique for making cell blocks is especially useful for cytologic samples with low cellularity, such as cerebrospinal fluid specimens.
细胞块切片作为细胞学涂片、液基 SurePath 细胞学和液基薄层细胞学检测（TCT）的重要诊断附件。文献中描述了多种制备细胞块的方法，细胞块技术在不断改进。本研究引入了一种新的制作细胞块的技术。我们首先使用预胶化淀粉作为细胞块的支架，这是一种非常简单和经济的方法，因为它可以在室温下进行，而无需额外的特殊仪器。我们已经在 122 例细胞学标本的细胞块切片中进行了苏木精和伊红（HE）染色、免疫组织化学分析和荧光原位杂交（FISH）。本文的结果表明，预胶化淀粉是一种有用的细胞块支架。预胶化淀粉可以有效地收集具有强大粘附力的少量细胞。因此，这种新的制作细胞块的技术对于细胞数量较少的细胞学标本，如脑脊液标本，特别有用。
