Amini Fatemeh Ghaed, Rafieian-Kopaei Mahmoud, Nematbakhsh Mehdi, Baradaran Azar, Nasri Hamid
Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
J Res Med Sci. 2012 Jul;17(7):621-5.
The aim of this study was to test the potential properties of metformin (MF) to protect the kidney from gentamicin (GM)-induced renal toxicity.
In this preclinical study, 50 male Wistar rats were randomly divided into five groups of 10 rats in each. In the first group (group I), they were kept in the same condition as others without receiving drugs for 10 days. In group II, the rats were injected intraperitoneally with 100 mg/kg/day of GM for 10 consecutive days. Group III rats received 100 mg/kg/day MF orally for 10 days. In group IV, the rats received GM (100 mg/kg; intraperitoneally) for 10 days and 100 mg/kg/day MF orally for the next 10 days. In the last group (group V), the rats received a combination of GM 100 mg/kg/day intraperitoneally and MF 100 mg/kg/day orally for 10 days simultaneously. Serum blood urea nitrogen (BUN) and creatinine (Cr) values were measured and renal tissues of the animals were processed for light microscope examination.
The levels of BUN in groups II, IV, and V, and also the serum level of Cr in groups II and V were increased significantly after the experiment. Furthermore, post-treatment with MF or co-treatment with MF could prevent the elevation of serum BUN and Cr induced by GM and also attenuates the damage score (P < 0.05).
MF may prevent or ameliorate GM-induced acute renal failure, and therefore it might be beneficial in patients under treatment with this medicine.
本研究旨在测试二甲双胍（MF）保护肾脏免受庆大霉素（GM）诱导的肾毒性的潜在特性。
在这项临床前研究中，50只雄性Wistar大鼠被随机分为五组，每组10只。第一组（I组），它们在与其他组相同的条件下饲养10天，不接受药物治疗。在II组中，大鼠连续10天腹腔注射100mg/kg/天的GM。III组大鼠口服100mg/kg/天的MF，持续10天。在IV组中，大鼠接受GM（100mg/kg；腹腔注射）10天，随后10天口服100mg/kg/天的MF。在最后一组（V组）中，大鼠同时接受腹腔注射100mg/kg/天的GM和口服100mg/kg/天的MF，持续10天。测量血清血尿素氮（BUN）和肌酐（Cr）值，并对动物的肾组织进行光学显微镜检查。
实验后，II、IV和V组的BUN水平以及II和V组的血清Cr水平均显著升高。此外，MF后处理或与MF联合处理可预防GM诱导的血清BUN和Cr升高，并减轻损伤评分（P<0.05）。
MF可能预防或改善GM诱导的急性肾衰竭，因此对接受该药物治疗的患者可能有益。
